A Comparative Study of Pygmy Hippopotamus (Choeropsis liberiensis) Karyotype by Cross-Species Chromosome Painting

The family Hippopotamidae is comprised of two genera with two living species, the common hippo ( Hippopotamus amphibius ) and the pygmy hippo ( Choeropsis liberiensis ). Unlike the common hippo, the karyotype of C. liberiensis has not yet been investigated via cross-species chromosome painting metho...

Full description

Saved in:
Bibliographic Details
Published in:Journal of mammalian evolution 2017-12, Vol.24 (4), p.465-474
Main Authors: Fröhlich, Jan, Kubickova, Svatava, Musilova, Petra, Cernohorska, Halina, Muskova, Helena, Rubes, Jiri
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Anthropology
Artificial chromosomes
Bacterial artificial chromosomes
Biomedical and Life Sciences
Choeropsis liberiensis
Comparative studies
DNA probes
Evolutionary Biology
Fluorescence in situ hybridization
Genomes
Hippopotamidae
Hippopotamus amphibius
Homology
Human Genetics
Hybridization
Life Sciences
Original Paper
Phylogeny
Species
X chromosomes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The family Hippopotamidae is comprised of two genera with two living species, the common hippo ( Hippopotamus amphibius ) and the pygmy hippo ( Choeropsis liberiensis ). Unlike the common hippo, the karyotype of C. liberiensis has not yet been investigated via cross-species chromosome painting methods. We established chromosomal homologies between the pygmy hippo, pig, and cattle by fluorescence in situ hybridization using whole chromosome, arm-specific, region specific, and bacterial artificial chromosome (BAC) probes. Probes from the 18 pig autosomes painted 45 conserved chromosomal segments in the pygmy hippo genome. The pygmy hippo and cattle homology map was deduced from our hybridization results of painting probes to pygmy hippo chromosomes with a combination of previously published dromedary hybridization data. On the pygmy hippo and cattle homology map, 29 cattle autosomes revealed 39 conservative segments on pygmy hippo chromosomes. For a more detailed structural analysis of genome rearrangements and X chromosome structure, we used cattle region specific and BAC probes. Our report demonstrates that cattle probes are useful not only in comparative studies within Ruminantia, but also in more phylogenetically distant Artiodactyla species.
ISSN:1064-7554
1573-7055
DOI:10.1007/s10914-016-9358-5