Comparison of in vitro haploid induction in balm (Melissa officinalis)

The generation of haploids in lemon balm ( Melissa officinalis L.) is the prerequisite for accelerated development of homozygous lines. Wide experiments to induce sporophytic development were arranged. Donor plants of lemon balm were established in a greenhouse, climate chambers and in vitro. For ha...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2016-09, Vol.126 (3), p.561-566
Main Authors: Kästner, Ute, Kittler, Johannes, Marthe, Frank
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Callus
Cell culture
Cellular structure
Cultivation
Culture media
Embryos
Greenhouses
In vitro methods and tests
Life Sciences
Melissa officinalis
Microspores
Ovaries
Ovules
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Pollen
Pretreatment
Research Note
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Summary:The generation of haploids in lemon balm ( Melissa officinalis L.) is the prerequisite for accelerated development of homozygous lines. Wide experiments to induce sporophytic development were arranged. Donor plants of lemon balm were established in a greenhouse, climate chambers and in vitro. For haploid induction, different variations of media and culture conditions e.g. pretreatment with cold and heat were tested. Sporophytic development of pollen was induced in anther and microspore culture. Callus developed sporadically in anther culture. Multi-cellular structures and microcallus generated from microspores. In vitro cultivated ovules became dark brown and somatic callus generated at the cut surface. Cultivation of ovaries maintained swelling on medium, but undeveloped embryos died.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-016-1007-4