Sequestration of CO2 into CaCO3 using Carbonic Anhydrase Immobilization on Functionalized Aluminum Oxide

To develop the technology for CO 2 sequestration, the free and immobilized carbonic anhydrase treatment was performed. The amount of dissolved CO 2 was increased by 3-fold after enzyme treatment of 5 ppm, and the amount of calcium carbonate increased linearly as the CaCl 2 was increased to 100 mM. T...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2019-07, Vol.55 (4), p.375-379
Main Authors: Lim, H. K., Kim, D. R., Hwang, I. T.
Format: Article
Language:English
Subjects:
Aluminum
Aluminum oxide
Aminopropyltriethoxysilane
Biochemistry
Biomedical and Life Sciences
Calcium
Calcium carbonate
Calcium chloride
Carbon dioxide
Carbon sequestration
Carbonic anhydrase
Carbonic anhydrases
Enzymes
Immobilization
Life Sciences
Medical Microbiology
Microbiology
Silicon
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Summary:To develop the technology for CO 2 sequestration, the free and immobilized carbonic anhydrase treatment was performed. The amount of dissolved CO 2 was increased by 3-fold after enzyme treatment of 5 ppm, and the amount of calcium carbonate increased linearly as the CaCl 2 was increased to 100 mM. To immobilize the carbonic anhydrase, an aluminum oxide carrier surface was functionalized with octadecyltrichlorosilane, 1H,1H,2H,2H-perfluoroctyltriethoxysilane, 3-aminopropyltriethoxysilane, 3-mercaptopropyl triethoxysilane, chlorotrimethylsilane 3-cyanopropyltrichlorosilane or 3-glycidyloxypropyltriethoxysilane. The best effect among these substances was revealed with 3-aminopropyltriethoxysilane; the efficacy of the immobilized carbonic anhydrase was at over 99%, and even when it was repeatedly used up to 40 times, the efficacy of the immobilized enzyme was more than 80%. The optimal conditions for the immobilized enzyme were 60 o C and pH 7.0.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683819040112