Construction of a bioinspired laccase-mimicking nanozyme for the degradation and detection of phenolic pollutants

[Display omitted] •An active site-inspired CH-Cu nanozyme with laccase-like activity is synthesized.•The CH-Cu nanozyme has superior activity and stability compared to natural enzyme.•The CH-Cu nanozyme has broad substrate specificity for phenolic pollutants.•The CH-Cu nanozyme maintains 82% of its...

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Bibliographic Details
Published in:Applied catalysis. B, Environmental Environmental, 2019-10, Vol.254, p.452-462
Main Authors: Wang, Jinghui, Huang, Renliang, Qi, Wei, Su, Rongxin, Binks, Bernard P., He, Zhimin
Format: Article
Language:English
Subjects:
Biomimetics
Bisphenols
Catalysis
Catalysts
Catalytic activity
Chlorophenol
Copper
Electron transfer
Environmental catalysis
Environmental degradation
Enzymes
Epinephrine
High temperature
Histidine
Laccase
Metal ions
Metal-organic complex
Metals
Mimicry
Nanomaterials
Nanotechnology
Nanozyme
Peptide
Peptides
Phenolic compounds
Phenols
Pollutants
Pollution detection
Recyclability
Smartphones
Substrates
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Summary:[Display omitted] •An active site-inspired CH-Cu nanozyme with laccase-like activity is synthesized.•The CH-Cu nanozyme has superior activity and stability compared to natural enzyme.•The CH-Cu nanozyme has broad substrate specificity for phenolic pollutants.•The CH-Cu nanozyme maintains 82% of its catalytic activity after twelve cycles.•The detection of epinephrine by a smart phone is established based on the CH-Cu nanozyme. Nanozymes, defined as nanomaterials with enzyme-like activity, have attracted extensive interest in both fundamental and applied research. Laccases are members of the multi-copper oxidases, which are utilized as green catalysts in the environmental catalysis and biochemical industry. In this paper, we report a facile strategy for the preparation of a new class of nanozyme (denoted as CH-Cu) with laccase-like activity inspired by the structure of the active site and the electron transfer pathway of laccase via the coordination of Cu+/Cu2+ with a cysteine (Cys)-histidine (His) dipeptide. The CH-Cu nanozymes exhibit excellent catalytic activity, recyclability and substrate universality and have a similar Km (Michaelis constant) and a higher vmax (maximum rate) than laccase at the same mass concentration. They are robust under a variety of conditions, such as extreme pH, high temperature, long-term storage and high salinity, which can cause severe loss in the catalytic activity of laccase. Higher efficacy of the CH-Cu nanozymes compared with laccase in the degradation of chlorophenols and bisphenols is also demonstrated in a batch reaction. Furthermore, a method for the quantitative detection of epinephrine by a smart phone is established based on the CH-Cu nanozymes. We believe that this nanozyme has promising applications in environmental catalysis and rapid detection and expect that combining key peptides as metal ligands with metal ions to mimic the structure of the catalytic center of a natural enzyme will be a general and important strategy for the design and synthesis of a new type of nanozyme that can be used in various applications.
ISSN:0926-3373
1873-3883
DOI:10.1016/j.apcatb.2019.05.012