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Application of an enzyme encapsulated metal-organic framework composite for convenient sensing and degradation of methyl parathion
[Display omitted] •A simple UV–vis absorbance based biosensor is reported for methyl parathion using organophosphate hydrolase enzyme immobilized Terbium-MOF.•The LOD of the sensing system is comparable or better that earlier reported OPH based biosensors for OPPs.•The MOF-enzyme composite exhibits...
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Published in: | Sensors and actuators. B, Chemical Chemical, 2019-07, Vol.290, p.267-274 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | [Display omitted]
•A simple UV–vis absorbance based biosensor is reported for methyl parathion using organophosphate hydrolase enzyme immobilized Terbium-MOF.•The LOD of the sensing system is comparable or better that earlier reported OPH based biosensors for OPPs.•The MOF-enzyme composite exhibits improved activity, stability and reusability towards both sensing and degradation of the pesticide.
Terbium-BTC (BTC = 1,2,4-Benzenetricarboxylic acid) metal-organic framework (MOF) has been employed for in-situ encapsulation of hexahistidine-tagged organophosphate hydrolase (OPH6His) enzyme. The enzymatic activity and stability of OPH6His were found remarkably enhanced after its encapsulation within the MOF. The potential of this enzyme-MOF composite (OPH6His@Tb-BTC) has been explored as a dual platform to simultaneously detect organophosphate pesticides (OPPs) and catalyze their degradation. The OPH6His@Tb-BTC exhibited improved enzymatic activity (by approximately 30%) and reusability (at least five cycles). It is demonstrated to be a highly sensitive and simple optical bioprobe for the detection of an OPP candidate, methyl parathion with a low limit of detection (2.6 nM). A less toxic metabolite was formed through catalytic conversion of methyl parathion (i.e., upon its incubation with OPH6His@Tb-BTC), further supporting the promising role of this composite in the remediation of pesticide-contaminated environments. |
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ISSN: | 0925-4005 1873-3077 |
DOI: | 10.1016/j.snb.2019.03.116 |