Temporal Parameters of p53-GFP Protein Transfer via Exosomes in Cocultured HEK293 and GFP-HEK293 Cells

In this study, we present a model for visualization of the exosome transfer of p53-GFP protein between cultured mammalian cells. The temporal parameters of the accumulation of fluorescent labeled protein in recipient cells have been analyzed. We employed HEK293 cells transfected with p53∆Y126-GFP pl...

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Bibliographic Details
Published in:Cell and tissue biology 2019-05, Vol.13 (3), p.188-197
Main Authors: Pantina, R. A., Varfolomeeva, E. Yu, Burdakov, V. S., Landa, S. B., Bayramukov, V. Yu, Kovalev, R. A., Filatov, M. V.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cell Biology
Exosomes
Green fluorescent protein
Life Sciences
Mammalian cells
p53 Protein
Proteins
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Summary:In this study, we present a model for visualization of the exosome transfer of p53-GFP protein between cultured mammalian cells. The temporal parameters of the accumulation of fluorescent labeled protein in recipient cells have been analyzed. We employed HEK293 cells transfected with p53∆Y126-GFP plasmid (a GFP clone) as donor of the exosomes, while the original HEK293 cell line was used as a recipient. Our results provide evidence that the transfer of the GFP protein from cell to cell is carried out via exosomes. It has been shown that the accumulation of this protein by the recipient cells in cocultures of these cells takes a prolonged time. The temporal parameters of this transfer differ between the cells within the same population.
ISSN:1990-519X
1990-5203
DOI:10.1134/S1990519X1903009X