The determination of stigmastadienes in vegetable oils. Results of collaborative studies and the standardized method

The development by collaborative study of a standardised method for the determination of the stigmastadienes content in vegetable oils is described. The procedure involves the isolation of the unsaponifiable matter in the oil ands its fractioning on a silica gel column. The fraction containing the s...

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Bibliographic Details
Published in:Pure and applied chemistry 1999-02, Vol.71 (2), p.349-359
Main Authors: Dobarganes, M. C., Cert, A., Dieffenbacher, A.
Format: Article
Language:English
Subjects:
Collaboration
Gas chromatography
Silica gel
Silicon dioxide
Vegetable oils
Vegetables
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Summary:The development by collaborative study of a standardised method for the determination of the stigmastadienes content in vegetable oils is described. The procedure involves the isolation of the unsaponifiable matter in the oil ands its fractioning on a silica gel column. The fraction containing the steroidal hydrocarbons is then analysed by capillary gas chromatography and the quantitative assessment made by the use of an internal standard /cholea-3,5-staddiene). The procedure is very sensitive allowing determination at levels of 0.01 - 4.0 mg/kg.
ISSN:0033-4545
1365-3075
DOI:10.1351/pac199971020349