Penetration Profile of Taurine in the Human Skin and Its Distribution in Skin Layers

Purpose To measure in vitro release of taurine from a semisolid standard formulation (amphiphilic cream, DAC) containing 1% taurine, a multi-layer membrane system was used. The content and distribution of taurine in different healthy skin layers (stratum corneum, epidermis and dermis) before (native...

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Bibliographic Details
Published in:Pharmaceutical research 2008-08, Vol.25 (8), p.1846-1850
Main Authors: da Silva, D. L. P., Thiago, S. B., Pessôa, F. A., Mrestani, Y., Rüttinger, H. H., Wohlrab, J., Neubert, R. H. H.
Format: Article
Language:English
Subjects:
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering and Bioengineering
Biomedicine
Calibration
Chemical Phenomena
Chemistry, Pharmaceutical
Chemistry, Physical
Diffusion Chambers, Culture
Drug dosages
General pharmacology
Humans
In Vitro Techniques
Measurement techniques
Medical Law
Medical sciences
Membranes
Pharmaceutical technology. Pharmaceutical industry
Pharmacology
Pharmacology. Drug treatments
Pharmacology/Toxicology
Pharmacy
Research Paper
Skin Absorption - physiology
Solubility
Taurine - administration & dosage
Taurine - chemistry
Taurine - pharmacokinetics
Transdermal medication
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Summary:Purpose To measure in vitro release of taurine from a semisolid standard formulation (amphiphilic cream, DAC) containing 1% taurine, a multi-layer membrane system was used. The content and distribution of taurine in different healthy skin layers (stratum corneum, epidermis and dermis) before (native taurine) and after application of the DAC cream were determined using capillary electrophoresis. Methods The release of taurine from the DAC cream was studied using a multilayer membrane system. Due to the high hydrophilic properties of taurine, the artificial model membranes consisted of collodion as matrix and glycerol as the acceptor phase. In order to determine whether taurine shows the potential for dermal penetration a Franz diffusion cell system was used. The distribution of taurine in the skin layers was determined before and after application of the DAC cream followed by the incubation in a Franz diffusion cell. The excised skin sample was cut in horizontal sections using a cryomicrotome. In order to detect taurine, fluorescamine was used as a derivatization agent. Results Experiments with a multilayer membrane system were performed to verify the release of taurine at different times (1, 2 and 5 h). Approximately 42.5% taurine was released from the semisolid standard formulation, accumulating in the first membrane (17.63%). The native taurine content was quantified in human isolated skin layer before and after the application of the semisolid standard formulation followed by incubation in a Franz-type diffusion cell for 1 and 5 h. No statistically significant difference ( p  
ISSN:0724-8741
1573-904X
DOI:10.1007/s11095-008-9589-0