Microbial exopolymer-capped selenium nanowires – Towards new antibacterial, antibiofilm and arbovirus vector larvicides?

Arboviral diseases and microbial pathogens resistant to commercially available drugs are on the rise. Herein, a facile microbial-based approach was developed to synthesize selenium nanowires (Se NWs) using microbial exopolymer (MEP) extracted from the Bacillus licheniformis (probiotic bacteria). MEP...

Full description

Saved in:
Bibliographic Details
Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2019-03, Vol.192, p.55-67
Main Authors: Abinaya, Muthukumar, Vaseeharan, Baskaralingam, Rekha, Ravichandran, Shanthini, Sivakumar, Govindarajan, Marimuthu, Alharbi, Naiyf S., Kadaikunnan, Shine, Khaled, Jamal M., Al-Anbr, Mohammed N.
Format: Article
Language:English
Subjects:
Aedes aegypti
Aquatic insects
Bacteria
Biofilms
Biomedical materials
Cytotoxicity
Dengue
Functional groups
Gram-negative bacteria
Iron
Larvae
Larvicides
Lymphatic filariasis
Microbial pathogens
Microorganisms
Microscopic analysis
Midgut
Mosquitoes
Nanobiotechnology
Nanoparticles, Zika virus
Nanotechnology
Nanowires
Pathogens
Probiotics
Selenium
Vectors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Arboviral diseases and microbial pathogens resistant to commercially available drugs are on the rise. Herein, a facile microbial-based approach was developed to synthesize selenium nanowires (Se NWs) using microbial exopolymer (MEP) extracted from the Bacillus licheniformis (probiotic bacteria). MEP-Se NWs were characterized using UV–Visible, XRD, FTIR, HR-TEM, FE-SEM and EDX. An UV–Visible peak was detected at 330 nm while XRD spectrum data pointed out the crystalline nature of MEP-Se NWs. FTIR spectrum revealed functional groups with strong absorption peaks in the range 3898.52–477.97 cm−1. FE-SEM and HR-TEM revealed that the obtained structures were nanowires of 10–30 nm diameter. Se presence was confirmed by EDX analysis. MEP-Se NWs at 100 μg/ml highly suppressed the growth of both Gram (−) and Gram (+) bacteria. Further, microscopic analysis evidenced that 75 μg/ml MEP-Se NWs suppressed biofilm formation. Hemolytic assays showed that MEP-Se NWs were moderately cytotoxic. In addition, LC50 values lower than 10 μg/ml were estimated testing MEP-Se NWs on both Aedes aegypti and Culex quinquefasciatus 3rd instar larvae. Morphological and histological techniques were used to elucidate on the damages triggered in mosquito tissues, with special reference to midgut, post-exposure to MEP-Se NWs. Therefore, based on our findings, MEP-Se NWs can be considered for entomological and biomedical applications, with special reference to the management of biofilm forming microbial pathogens and arbovirus mosquito vectors. [Display omitted] •Se nanowires (Se NWs) were synthesized using microbial exopolymers (MEP) from Bacillus licheniformis Dahb1.•MEP-Se NWs were characterized by UV–Vis, XRD, FTIR, FE-SEM, HR-TEM and EDX.•MEP-Se NWs revealed great antioxidant and antibiofilm activity.•Low hemolytic activity of MEP-Se NWs pointed out their biocompatible nature.•MEP-Se NWs were highly toxic to Aedes aegypti and Culex quinquefasicatus larvae.
ISSN:1011-1344
1873-2682
DOI:10.1016/j.jphotobiol.2019.01.009