A sensitive electrochemiluminescence immunosensor for the detection of PSA based on CdWS nanocrystals and Ag+@UIO-66-NH2 as a novel coreaction accelerator

In this work, an effective sandwich electrochemiluminescence (ECL) immunosensing scheme was successfully constructed for the determination of prostate specific antigen (PSA). Specifically, self-prepared dual-stabilizers-capped CdWS nanocrystals (NCs) was integrated onto Ag+@UIO-66-NH2 which is a nov...

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Bibliographic Details
Published in:Electrochimica acta 2019-04, Vol.302, p.207-215
Main Authors: Fang, Qingcheng, Lin, Zhihong, Lu, Fushen, Chen, Yaowen, Huang, Xiaochun, Gao, Wenhua
Format: Article
Language:English
Subjects:
Antigens
Biosensors
CdWS nanocrystals
Coreaction accelerator
Electrochemiluminescence
Electrochemiluminescence immunosensor
Graphene
Immunosensors
Linearity
Metal-organic frameworks
Nanocrystals
Prostate
PSA
Sensitivity analysis
Silver
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Summary:In this work, an effective sandwich electrochemiluminescence (ECL) immunosensing scheme was successfully constructed for the determination of prostate specific antigen (PSA). Specifically, self-prepared dual-stabilizers-capped CdWS nanocrystals (NCs) was integrated onto Ag+@UIO-66-NH2 which is a novel coreaction accelerator for improving ECL intensity of the CdWS/S2O82− system, to create a Ag+@UIO-66-NH2@CdWS ECL nanoprobes for increasing the analysis sensitivity efficiently. Reduced graphene oxide (rGO) and Au NPs were modified on the surface of GCE to set up a stable sensitive sensing area, on which PSA capture antibody (Ab1) was immobilized to capture PSA via antigen-antibody immunoreaction. Then Ag+@UIO-66-NH2@CdWS labeled antibody (Ab2) was used as signal probe. Under the optimal conditions, the ECL intensity presented an excellent linear relationship with the logarithm of PSA concentration from 0.0001 to 10 ng mL−1, with a low detection limit of 0.038 pg mL−1. This indicated that the designed ECL biosensor is valuable, innovative, and wide linearity to the detection of PSA, which provides a worthy method in clinical diagnosis. More importantly, this work provided CdWS NCs luminophor and Ag+@UIO-66-NH2 coreaction accelerator, which improve the sensitivity of goal analysis in the field of ECL biosensor. A sandwich ECL immunosensor was constructed based on using the Ag+@UIO-66-NH2@CdWS as ECL probes for the quantitative evaluation of PSA. rGO and Au NPs were modified onto the surface of GCE electrode, on which Ab1 was immobilized to capture PSA. After that, Ab2 labeled Ag+@UIO-66-NH2@CdWS was used to recognize the captured PSA. [Display omitted] •The CdWS nanocrystals (NCs) as efficient ECL emitters was first synthesized using dual-stabilizers-capped strategy.•Ag+@UIO-66-NH2 could act as a novel coreaction accelerator towards the CdWS/S2O82− system.•Expanding a new application of Ag+@UIO-66-NH2 in ECL field.•The Ag+@UIO-66-NH2/CdWS/S2O82− ternary system was initially constructed, and its ECL mechanism was preliminarily explored.•A sandwich-configuration ECL immunosensor for the detection of PSA was developed.
ISSN:0013-4686
1873-3859
DOI:10.1016/j.electacta.2019.02.027