In vivo antitumor effect of endostatin-loaded chitosan nanoparticles combined with paclitaxel on Lewis lung carcinoma

The purpose of this study was to prepare endostatin-loaded chitosan nanoparticles (ES-NPs) and evaluate their antitumor effect when combined with paclitaxel (PTX) on Lewis lung carcinoma (LLC) mouse xenografts. ES-NPs were prepared by ionic cross-linking. Characterization of the ES-NPs included size...

Full description

Saved in:
Bibliographic Details
Published in:Drug delivery 2017, Vol.24 (1), p.1410-1418
Main Authors: Xie, Fang, Ding, Rui-Lin, He, Wen-Feng, Liu, Zong-Jun-Lin, Fu, Shao-Zhi, Wu, Jing-Bo, Yang, Ling-Lin, Lin, Sheng, Wen, Qing-Lian
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Cancer therapies
Carcinoma, Lewis Lung
Cell Line, Tumor
Chemotherapy
Chitosan
Endostatin
Endostatins
Kinases
Lung cancer
Mice
Mice, Inbred C57BL
Nanoparticles
Paclitaxel
Peptides
Proteins
Tumors
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The purpose of this study was to prepare endostatin-loaded chitosan nanoparticles (ES-NPs) and evaluate their antitumor effect when combined with paclitaxel (PTX) on Lewis lung carcinoma (LLC) mouse xenografts. ES-NPs were prepared by ionic cross-linking. Characterization of the ES-NPs included size distribution, drug-loading efficiency (DL), and encapsulation efficiency (EE). An in vitro release test was also used to determine the release behavior of the ES-NPs. A subcutaneous LC xenograft model of C57BL/6J mice was established. The mice were randomly divided into six groups: control (0.9% NaCl), ES, PTX, ES-NPs, ES + PTX, and ES-NPs + PTX. The tumor volume was dynamically measured for the duration of the experiment. Immunohistochemistry was performed to determine the Ki-67 and microvascular density (MVD) in each group. Serum vascular endothelial growth factor (VEGF) and ES levels were determined by enzyme-linked immunosorbent assay (ELISA). ES-NPs were successfully synthesized and had suitable size distribution and high EE. The NPs were homogenously spherical and exhibited an ideal release profile in vitro. In vivo, tumor growth was significantly inhibited in the ES-NPs + PTX group. The tumor inhibitory rate was significantly higher in the ES-NPs + PTX group than in the other groups (p 
ISSN:1071-7544
1521-0464
DOI:10.1080/10717544.2017.1378938