The Screening Research of NF-κB Inhibitors from Moutan Cortex Based on Bioactivity-Integrated UPLC-Q/TOF-MS

Inflammation is a common and important pathological process, and nuclear factor-κB (NF-κB) is a key mediator of it. Moutan Cortex (MC), the dried root cortex of Paeonia suffruticosa Andr., is widely used as a remedy for the treatment of inflammatory diseases in Asian region. However, there are few s...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2019-01, Vol.2019 (2019), p.1-7
Main Authors: Jiang, Min, Deng, Yanfang, Zhang, Man, Liu, Wenjuan, Lu, Yujie, Bai, Gang
Format: Article
Language:English
Subjects:
Acids
Activation
Anti-inflammatory agents
Arthritis
Biological activity
Cancer therapies
Cardiovascular disease
Cell culture
Chinese medicine
Cytokines
Drugs
Gallic acid
Herbal medicine
Inflammation
Inflammatory diseases
Inhibitors
Liquid chromatography
Lung cancer
Mass spectrometry
Mass spectroscopy
Medical research
Medical treatment
NF-κB protein
Pharmaceuticals
Quadrupoles
Tumor necrosis factor-TNF
Tumor necrosis factor-α
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Summary:Inflammation is a common and important pathological process, and nuclear factor-κB (NF-κB) is a key mediator of it. Moutan Cortex (MC), the dried root cortex of Paeonia suffruticosa Andr., is widely used as a remedy for the treatment of inflammatory diseases in Asian region. However, there are few studies on the systematic identification of NF-κB inhibitors of MC. In this study, the effect of inhibiting NF-κB activation of MC was assessed at the cellular level using a tumor necrosis factor-α (TNF-α) induced inflammatory model. Subsequently, ultra-performance liquid chromatography-quadrupole/time of flight-mass spectrometry (UPLC-Q/TOF-MS) combined with biological activity assay was established to screen and identify potential anti-inflammatory ingredients in MC. The results revealed that MC significantly inhibited the activation of NF-κB. Seven potential NF-κB inhibitors were screened from MC, including oxypaeoniflorin, paeoniflorin, galloylpaeoniflorin, benzoyloxypaeoniflorin, mudanpioside C, gallic acid, and paeonol. Among them, the NF-κB inhibitor activity of galloylpaeoniflorin, benzoyloxypaeoniflorin, and mudanpioside C is first reported here. In conclusion, the anti-inflammatory activity of MC was associated with the seven components mentioned above. And the bioactivity-integrated UPLC-Q/TOF which contains both chemical and bioactive details is suitable for screening active ingredients from natural medicines.
ISSN:1741-427X
1741-4288
DOI:10.1155/2019/6150357