In vitro formation of holes on the inner perivitelline layer of quail ovum by chicken spermatozoa

ABSTRACT The aim of the present study was to examine whether chicken semen can be substituted for quail semen to conduct in vitro experiments on fertilization. Chicken spermatozoa was incubated with the inner perivitelline layer (IPL) isolated from the largest follicle in the quail ovary under in vi...

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Bibliographic Details
Published in:Animal science journal 2005-04, Vol.76 (2), p.133-138
Main Authors: WIN, Mar Mar, MIYAGI, Yuki, ASHIZAWA, Koji, TATEMOTO, Hideki, NAKADA, Tadashi
Format: Article
Language:English
Subjects:
Birds
Body fluids
chicken spermatozoa
in vitro perforation
inner perivitelline layer
Poultry
quail ovum
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Summary:ABSTRACT The aim of the present study was to examine whether chicken semen can be substituted for quail semen to conduct in vitro experiments on fertilization. Chicken spermatozoa was incubated with the inner perivitelline layer (IPL) isolated from the largest follicle in the quail ovary under in vitro conditions. The perforation of chicken and quail spermatozoa were assessed by counting the number of all visible holes in the pieces of IPL. No difference was found in the number of holes formed by the chicken sperm and quail IPL interaction compared with that between intraspecies. In addition, the number of holes in the IPL was significantly increased with the increase in sperm concentration from 1 × 106 to 8 × 106 sperm/mL (P 
ISSN:1344-3941
1740-0929
DOI:10.1111/j.1740-0929.2005.00248.x