The preparation and antibacterial effect of egg yolk immunoglobulin (IgY) against the outer membrane proteins of Vibrio parahaemolyticus

BACKGROUND Vibrio parahaemolyticus causes not only various diseases in aquaculture animals but also seafood‐borne illness in humans. Outer membrane proteins (OMPs) are species‐specific proteins found in bilayer membranes of gram‐negative bacteria. Egg yolk immunoglobulin (IgY) has been reported to s...

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Bibliographic Details
Published in:Journal of the science of food and agriculture 2019-03, Vol.99 (5), p.2565-2571
Main Authors: Hu, Bochao, Yang, Xiaodong, Guo, Erpeng, Zhou, Peizhan, Xu, Ding, Qi, Zhenqiang, Deng, Li
Format: Article
Language:English
Subjects:
Antibacterial materials
Antibodies
anti‐bacterial effect
Aquaculture
Bacteria
Decapoda
Diet
E coli
egg yolk immunoglobulin (IgY)
Extrusion
Gram-negative bacteria
Immunity
Immunity (passive)
Immunization
Immunoglobulins
In vivo methods and tests
Membrane proteins
Membranes
Muscles
Oral administration
Organic chemistry
Outer membrane proteins
Proteins
Seafood
Superoxide dismutase
Vibrio parahaemolyticus
Viruses
Waterborne diseases
Yolk
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Summary:BACKGROUND Vibrio parahaemolyticus causes not only various diseases in aquaculture animals but also seafood‐borne illness in humans. Outer membrane proteins (OMPs) are species‐specific proteins found in bilayer membranes of gram‐negative bacteria. Egg yolk immunoglobulin (IgY) has been reported to serve as oral administration of antibodies against bacteria and virus. RESULTS The present research extracted and identified OMPs from V. parahaemolyticus, and then the extracted OMPs were used to immunize hens to obtain specific IgY. The efficacy of IgY against V. parahaemolyticus were investigated in vitro and in vivo. The specific IgY effectively inhibited the growth of V. parahaemolyticus in liquid medium rather than Escherichia coli and Staphylococcus aureus. Specific IgY antibodies were incorporated into extruded food pellets and fed to bacteria‐challenged white pacific shrimp to observe the anti‐bacterial effect in vivo. The bacterial loads in muscles of V. parahaemolyticus infected shrimp fed with specific IgY‐included diets were significantly fewer than those fed with non‐specific IgY‐included diets. The superoxide dismutase activities in muscles of infected shrimp fed with specific IgY‐included diets were significantly higher than the control group. CONCLUSION The results suggested that the specific IgY effectively inhibited the growth of V. parahaemolyticus and introduced passive immunity to shrimp. © 2018 Society of Chemical Industry
ISSN:0022-5142
1097-0010
DOI:10.1002/jsfa.9470