Novel bioelectrodes based on polysaccharide modified gold surfaces and electrochemically active Lactobacillus rhamnosus GG biofilms

Lactobacillus rhamnosus GG is an environmentally and human-friendly bacteria that can be found in probiotics. However, it is incapable of forming a biofilm on metal surfaces, what limits the possibility of using it in bioelectrochemical systems (BESs) as promising and green alternatives. Hence, we p...

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Bibliographic Details
Published in:Electrochimica acta 2019-02, Vol.296, p.999-1008
Main Authors: Jarosz, Magdalena, Grudzień, Joanna, Kamiński, Kamil, Gawlak, Karolina, Wolski, Karol, Nowakowska, Maria, Sulka, Grzegorz D.
Format: Article
Language:English
Subjects:
Atomic beam spectroscopy
Atomic force microscopy
Bioelectrochemical systems
Biofilm formation
Biofilms
Cationic dextran derivative
Electrodes
Gold
Infrared spectroscopy
Lactobacilli
Lactobacillus rhamnosus GG
Metal surfaces
Metal/polycation electrodes
Polyelectrolytes
Polysaccharides
Probiotics
Self-assembly
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Summary:Lactobacillus rhamnosus GG is an environmentally and human-friendly bacteria that can be found in probiotics. However, it is incapable of forming a biofilm on metal surfaces, what limits the possibility of using it in bioelectrochemical systems (BESs) as promising and green alternatives. Hence, we propose a simple and cost-effective modification of metal surface with cationic dextran derivative (DEX-G) by using the electrostatically driven self-assembly procedure resulting in the formation of a polymeric monolayer. Modification of metal electrodes was confirmed by using infrared spectroscopy (IR) and atomic force microscopy (AFM). Synthesized metal/polycation electrodes provided a bioactive environment for L. rhamnosus GG enhancing its proliferation and formation of the biofilm as confirmed by SEM imaging. Moreover, using cyclic voltammetry (CV) and chronoamperometry measurements, it was proven that metal/polycation/L. rhamnosus bioelectrodes are electrochemically active in the glucose-rich medium. The electrochemical activity of fabricated bioanodes allows to consider them for their potential use in the bioelectrochemical systems. [Display omitted] •Metal surface modification method facilitating the biofilm formation is proposed.•Use of lactic acid bacteria for bioelectrochemical systems is examined.•Cationic dextran coating enhanced bacteria colonization of gold.•The electroactivity of proposed bioanodes was confirmed by cyclic voltammetry.
ISSN:0013-4686
1873-3859
DOI:10.1016/j.electacta.2018.11.154