Development of high-sensitivity ion trap ion mobility spectrometry time-of-flight techniques: A high-throughput nano-LC-IMS-TOF separation of peptides arising

A linear octopole trap interface for an ion mobility timeof-flight mass spectrometer has been developed for focusing and accumulating continuous beams of ions produced by electrospray ionization. The interface improves experimental efficiencies by factors of -50-200 compared with an analogous config...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2003-10, Vol.75 (19), p.5137
Main Authors: Myung, Sunnie, Young Jin Lee, Moon, Myeong Hee, Taraszka, John
Format: Article
Language:English
Subjects:
Ions
Peptides
Sensitivity
Spectrum analysis
Online Access:Get full text
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Summary:A linear octopole trap interface for an ion mobility timeof-flight mass spectrometer has been developed for focusing and accumulating continuous beams of ions produced by electrospray ionization. The interface improves experimental efficiencies by factors of -50-200 compared with an analogous configuration that utilizes a three-dimensional Paul geometry trap (Hoaglund-Hyzer, C. S.; Lee, Y. J.; Counterman, A. E.; Clemmer, D. E. AnaL ChenL 2002, 74, 992-1006). With these improvements, it is possible to record nested drift (flight) time distributions for complex mixtures in fractions of a second. We demonstrate the approach for several well-defined peptide mixtures and an assessment of the detection limits is given. Additionally, we demonstrate the utility of the approach in the field of proteomics by an on-line, threedimensional nano-LC-ion mobility-TOF separation of tryptic peptides from the Drosophila proteome. [PUBLICATION ABSTRACT]
ISSN:0003-2700
1520-6882