Heterologous overexpression of Lithospermum erythrorhizon LeERF-1 gene increases drought and pathogen resistance in Arabidopsis

Ethylene-responsive transcription factors (ERFs) belonging to the AP2/ERF family have diverse functions in plants. However, the functions of LeERF-1 , a member of ERF family from the medicinal plant Lithospermum erythrorhizon , remain unclear. In this study, by cloning the promoter of LeERF-1 , we f...

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Bibliographic Details
Published in:Acta physiologiae plantarum 2019-02, Vol.41 (2), Article 24
Main Authors: Fang, Rong-Jun, Li, Ai-Qian, Tian, Ru-Nan, Zhang, Wen-Ju, Zou, Ai-Lan, Wu, Feng-Yao, Liao, Yong-Hui, Wang, Xiao-Ming, Pang, Yan-Jun, Yang, Rong-Wu, Lu, Gui-Hua, Qi, Jin-Liang, Yang, Yong-Hua
Format: Article
Language:English
Subjects:
Agriculture
Apertures
Arabidopsis
Biomedical and Life Sciences
Cloning
Drought
Genetic engineering
Herbal medicine
Inoculation
Leaves
Life Sciences
Lithospermum erythrorhizon
Medicinal plants
Original Article
Pathogens
Phenotypes
Plant Anatomy/Development
Plant Biochemistry
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Signs and symptoms
Stomata
Transcription factors
Water loss
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Summary:Ethylene-responsive transcription factors (ERFs) belonging to the AP2/ERF family have diverse functions in plants. However, the functions of LeERF-1 , a member of ERF family from the medicinal plant Lithospermum erythrorhizon , remain unclear. In this study, by cloning the promoter of LeERF-1 , we found that the promoter region contained a number of potential regulatory motifs related to drought and pathogen resistances. Further transgenic studies showed that the heterologous overexpression of LeERF-1 in Arabidopsis displayed phenotypes of higher survival ratio, lower root inhibition rate, slow water loss in leaf discs, and smaller stomatal apertures under drought stress, compared with wild type (WT) of Arabidopsis. LeERF-1 transgenic Arabidopsis also displayed fewer chlorotic symptoms, lower incidence rates, and lower levels of bacterial proliferation on leaves after the inoculation of bacterial pathogen compared with WT. These results suggested that LeERF-1 can also confer drought and pathogen resistances. Our work provided a candidate gene with remarkable potential use in genetic engineering for stress resistance improvement in plants.
ISSN:0137-5881
1861-1664
DOI:10.1007/s11738-019-2807-z