Activatable cell-biomaterial interfacing with photo-caged peptides

Spatio-temporally tailoring cell-material interactions is essential for developing smart delivery systems and intelligent biointerfaces. Here we report new photo-activatable cell-material interfacing systems that trigger cellular uptake of various cargoes and cell adhesion towards surfaces. To achie...

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Bibliographic Details
Published in:Chemical science (Cambridge) 2019-01, Vol.10 (4), p.1158-1167
Main Authors: Lin, Yiyang, Mazo, Manuel M, Skaalure, Stacey C, Thomas, Michael R, Schultz, Simon R, Stevens, Molly M
Format: Article
Language:English
Subjects:
Biomedical materials
Cell adhesion
Cell adhesion & migration
Chemical compounds
Fluorescence
Gold
Ligands
Liposomes
Nanomaterials
Nanoparticles
Peptides
Proteins
Quantum dots
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Summary:Spatio-temporally tailoring cell-material interactions is essential for developing smart delivery systems and intelligent biointerfaces. Here we report new photo-activatable cell-material interfacing systems that trigger cellular uptake of various cargoes and cell adhesion towards surfaces. To achieve this, we designed a novel photo-caged peptide which undergoes a structural transition from an antifouling ligand to a cell-penetrating peptide upon photo-irradiation. When the peptide is conjugated to ligands of interest, we demonstrate the photo-activated cellular uptake of a wide range of cargoes, including small fluorophores, proteins, inorganic ( , quantum dots and gold nanostars) and organic nanomaterials ( , polymeric particles), and liposomes. Using this system, we can remotely regulate drug administration into cancer cells by functionalizing camptothecin-loaded polymeric nanoparticles with our synthetic peptide ligands. Furthermore, we show light-controlled cell adhesion on a peptide-modified surface and 3D spatiotemporal control over cellular uptake of nanoparticles using two-photon excitation. We anticipate that the innovative approach proposed in this work will help to establish new stimuli-responsive delivery systems and biomaterials.
ISSN:2041-6520
2041-6539
DOI:10.1039/c8sc04725a