Integrating MFT-qPCR techniques in constructed wetland faecal bacterial purification monitoring; a case of a typical tropical hybrid constructed wetland system

The sanitation control of pathogens in the tropical effluents needs much more attention to ensure ecosystem health integrity and the safety of human health. The common use of chemicals in achieving this in wastewater treatment has remained unsustainable due to much health concern. Indeed, based on t...

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Bibliographic Details
Published in:Water science and technology 2018-12, Vol.78 (9), p.2008-2018
Main Authors: Omondi, Donde Oscar, Wairimu, Muia Anastasia, Maingi, Makindi Stanley, Otieno, Onyango Godfrey, Jepkorir, Kibet Caroline, Okoth, Ogalo Joseph, Bangding, Xiao
Format: Article
Language:English
Subjects:
Aquatic plants
Artificial wetlands
Bacteria
Climate change
Ecosystems
Efficiency
Effluents
Environmental conditions
Environmental health
Environmental science
Health
Human wastes
Humans
Hybrid systems
Laboratories
Membrane filtration
Microorganisms
Molecular chains
Monitoring
Nucleotide sequence
Organic chemistry
Pathogenic bacteria
Pathogens
PCR
Polymerase chain reaction
Purification
Sanitation
Tropical climate
Waste Disposal, Fluid - methods
Waste Water
Wastewater
Wastewater treatment
Water Purification
Water treatment
Wetlands
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Summary:The sanitation control of pathogens in the tropical effluents needs much more attention to ensure ecosystem health integrity and the safety of human health. The common use of chemicals in achieving this in wastewater treatment has remained unsustainable due to much health concern. Indeed, based on the numerous challenges associated with faecal pathogenic bacteria in wastewaters, the focus is now on achieving higher purification efficiencies in the elimination of the human pathogens from wastewater through eco-sustainable systems such as constructed wetlands (CWs). Hence, the need to explore the application of constructed wetlands in wastewater treatment under specific local environmental conditions for accurate understanding and improved treatment efficiency. This study therefore aimed at monitoring constructed wetlands faecal bacteria purification efficiency through integrated non-molecular membrane filtration technique and molecular quantitative polymerase chain reaction (MFT-qPCR) technique. The results showed some shortfall in the treatment system and also proved that integrating MFT-qPCR in faecal bacterial purification monitoring within a constructed wetland system provides a more accurate and reliable outcome. Additionally, the wetland purification efficiency was low (
ISSN:0273-1223
1996-9732
DOI:10.2166/wst.2018.475