Enzymatic treatments as alternative to produce chitin fragments of low molecular weight from Alternaria alternata

ABSTRACT Chitin and its oligosaccharides are involved in the plant defense response against fungal pathogens. In most studies, these molecules come from crustacean shell, and there are scarce studies on the use of fungal chitin. Usually the extraction of chitin is by alkaline treatments, which affec...

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Published in:Journal of applied polymer science 2019-04, Vol.136 (15), p.n/a
Main Authors: Henry García, Yaima, Troncoso‐Rojas, Rosalba, Tiznado‐Hernández, Martín Ernesto, Báez‐Flores, María Elena, Carvajal‐Millan, Elizabeth, Rascón‐Chu, Agustín, Lizardi‐Mendoza, Jaime, Martínez‐Robinson, Karla Guadalupe
Format: Article
Language:English
Subjects:
Acetylation
Alternaria alternata
Chitin
Crustaceans
Fragmentation
Fragments
FT‐IR
fungal chitin fragments
Fungi
glucanase treatments
Low molecular weights
Materials science
Molecular weight
Oligosaccharides
Polymers
protease treatments
Proteins
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Summary:ABSTRACT Chitin and its oligosaccharides are involved in the plant defense response against fungal pathogens. In most studies, these molecules come from crustacean shell, and there are scarce studies on the use of fungal chitin. Usually the extraction of chitin is by alkaline treatments, which affect the acetylation degree (DA), and the obtaining of oligosaccharides of low molecular weight; so the use of enzymes is proposed as an alternative treatment to obtain chitin oligosaccharides from fungi. The objective of this work was to characterize the chitin fragments of Alternaria alternata extracted by alkaline and enzymatic treatments. The chitin extracted was ultrasonicated and ultrafiltrated to produce chitin fragments. Enzymatic treatments decreased the protein content to 29%, and the bands corresponding to β‐glucans decreased when β‐1,3‐glucanase was applied. Fragments smaller than 1 kDa, DA of 76.7%, and 35.4 μg glucosamine/mg dry weight were obtained by enzymatic treatments. The enzymatic treatments show promising results to extract chitin fragments from A. alternata without severely affect the DA of the molecule. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47339.
ISSN:0021-8995
1097-4628
DOI:10.1002/app.47339