Antileishmanial and antioxidant potential of fractions and isolated compounds from Nectandra cuspidata

The effects on the viability of promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and citotoxicity in J774.A1 macrophages of ethanolic extract, fractions and isolated compounds that were obtained from the leaves of Nectandra cuspidata were determined. The total phenolics conte...

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Published in:Natural product research 2018-12, Vol.32 (23), p.2825-2828
Main Authors: Ferreira, Lilian dos Anjos Oliveira, Oliveira, Mariana Maciel de, Faleiro, Flávia Lais, Scariot, Débora Botura, Boeing, Joana Schuelter, Visentainer, Jesuí Vergílio, Romagnolo, Mariza Barion, Nakamura, Celso Vataru, Truiti, Maria da Conceição Torrado
Format: Article
Language:English
Subjects:
Acetic acid
Animals
Antioxidants
Antioxidants - isolation & purification
Antioxidants - pharmacology
Antiprotozoal Agents - chemistry
Antiprotozoal Agents - isolation & purification
Antiprotozoal Agents - pharmacology
Butylated hydroxytoluene
Cell Line
Cytotoxicity
Epicatechin
Ethyl acetate
lauraceae
Lauraceae - chemistry
Leishmania
Leishmania - drug effects
Leishmaniasis
Macrophages
Macrophages - drug effects
medicinal plant
Mice
Nectandra
oxidative stress
Phenols - analysis
Plant Extracts - chemistry
Plant Extracts - pharmacology
Quercetin
Toxicity
Viability
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Summary:The effects on the viability of promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and citotoxicity in J774.A1 macrophages of ethanolic extract, fractions and isolated compounds that were obtained from the leaves of Nectandra cuspidata were determined. The total phenolics content (TP) and antioxidant activity were assessed using different assays. The ethyl acetate fraction (EAF) had the best antiproliferative activity (amastigote IC 50 : 4.42 ± 0.60 μg/mL, promastigote IC 50 : 33.33 ± 0.78) and presented no cytotoxicity at the highest concentration tested (1000 μg/mL). The EAF also had the greatest antioxidant capacity, which was comparable to butylated hydroxytoluene and quercetin (DPPH IC 50 6.54 ± 0.10 μg/mL, ABTS 4.75 ± 0.11 mmol ET/g; FRAP 2.37 ± 0.03 mmol ET/g; ORAC = 33.52 ± 1.99 mmol ET/g; TP 387.11 ± 0.25 mg GAE/g). Vitexin, epicatechin and isovitexin isolated from EAF contribute to both activities.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2017.1378214