Leptin impairs the therapeutic effect of ionizing radiation in oral squamous cell carcinoma cells

Purpose Leptin, an important hormone controlling energy homeostasis, has been linked to the pathogenesis of oral squamous cell carcinoma (OSCC). Evidence indicates that head and neck cancer patients undergoing radiotherapy show decreased leptin levels after radiotherapy treatment. Thus, we investiga...

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Bibliographic Details
Published in:Journal of oral pathology & medicine 2019-01, Vol.48 (1), p.17-23
Main Authors: da Rocha, Rogério Gonçalves, Santos, Eliane Macedo Sobrinho, Santos, Eloá Mangabeira, Gomes, Emisael Stênio Batista, Ramos, Guilherme Veloso, Aguiar, Karina Marini, Gonçalves, Bruno Rodrigues, Santos, Sérgio Henrique Sousa, De Paula, Alfredo Maurício Batista, Guimarães, André Luiz Sena, Farias, Lucyana Conceição
Format: Article
Language:English
Subjects:
ACTC1
Carcinogenesis
Cell adhesion & migration
Cell death
Cell migration
Cell proliferation
Colonies
EEF2
Energy balance
Head & neck cancer
Homeostasis
Ionizing radiation
KRT6A
Leptin
Mass spectroscopy
Oral cancer
Oral squamous cell carcinoma
Phenotypes
Proteomes
Radiation therapy
Reactive oxygen species
Squamous cell carcinoma
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Summary:Purpose Leptin, an important hormone controlling energy homeostasis, has been linked to the pathogenesis of oral squamous cell carcinoma (OSCC). Evidence indicates that head and neck cancer patients undergoing radiotherapy show decreased leptin levels after radiotherapy treatment. Thus, we investigated, through phenotypic and molecular analyses, whether leptin can compromise the therapeutic effect of ionizing radiation and neoplastic behavior of OSCC cells. Methods The human OSCC‐derived cell lines SCC9 and SCC4 were treated with human recombinant leptin and exposed to 6 Gy of irradiation. We performed the in vitro assays of cell migration, death, proliferation, and colony‐forming ability. The reactive oxygen species (ROS) levels and proteome analysis by mass spectrometry were also conducted. Results Leptin was able to increase cell proliferation, migration, and colony‐forming ability, despite the suppressive effect induced by irradiation. Furthermore, the leptin promoted a significant reduction of ROS intracellular accumulation, and increased expression of the cancer‐related proteins, as ACTC1, KRT6A, and EEF2 in irradiated OSCC cells. Conclusions Our findings suggest that leptin impairs responsivity of OSCC cells to the ionizing radiation, reducing the suppressive effects of irradiation on the neoplastic phenotype, and increasing protein expression critical to carcinogenesis.
ISSN:0904-2512
1600-0714
DOI:10.1111/jop.12786