Phylogenetics and diversity analysis of Pennisetum species using Hemarthria EST‐SSR markers

Expressed sequence tag‐simple sequence repeat (EST‐SSR) markers are widely applied in plant molecular genetics studies due to their abundance in the genome, codominant nature, and high repeatability. To study the genetic diversity of 35 accessions and transferability of EST‐SSR markers in cross‐spec...

Full description

Saved in:
Bibliographic Details
Published in:Grassland science 2019-01, Vol.65 (1), p.13-22
Main Authors: Zhou, Sifan, Wang, Chengran, Yin, Guohua, Zhang, Yu, Shen, Xiaoyun, Pennerman, Kayla K., Zhang, Jianbo, Yan, Haidong, Zhang, Chenglin, Zhang, Xinquan, Ren, Shuping, Guo, Tianfeng, Peng, Yan, Ma, Xiao, Liu, Wei, Yan, Yanhong, Huang, Linkai
Format: Article
Language:English
Subjects:
Biodiversity
Cluster analysis
EST‐SSR
Expressed sequence tags
Gene polymorphism
Genetic diversity
Genetics
Genomes
Germplasm
Hemarthria
Markers
Pennisetum
Phylogeny
Polymorphism
Population studies
Species
Species diversity
transferability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Expressed sequence tag‐simple sequence repeat (EST‐SSR) markers are widely applied in plant molecular genetics studies due to their abundance in the genome, codominant nature, and high repeatability. To study the genetic diversity of 35 accessions and transferability of EST‐SSR markers in cross‐species applications, 21 primer pairs previously developed in Hemarthria were amplified across Pennisetum species. A total of 148 polymorphisms were generated with an average of 7.05 bands per locus. The mean values of the genetic parameters polymorphism information content, number of bands, effective number of bands, Nei's gene diversity, and Shannon's information index were 0.8430, 2.0000, 1.7640, 0.4247 and 0.6132, respectively. Cluster analysis of 35 accessions divided them into three clusters, which were consistent with dendrograms of 14 species. The among‐population component explained most of the genetic diversity (66%); the remaining variation (34%) occurred within populations. This study will provide more available EST‐SSR markers for Pennisetum species, and facilitate studies on germplasm collection and utilization of Pennisetum species.
ISSN:1744-6961
1744-697X
DOI:10.1111/grs.12208