In ovulo embryo development and plant formation from stenospermic genotypes of Vitis vinifera

Embryo enlargement and plant formation via in ovulo embryo culture was studied in stenospermic grapes Genotypes, culture media and their interactions significantly affected embryo enlargement, whereas culture dates did not. Genotype P60-58, when compared to cv Thompson Seedless, had the greatest num...

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Bibliographic Details
Published in:American journal of botany 1989-03, Vol.76 (3), p.397-402
Main Authors: Emershad, Richard L., Ramming, David W., Serpe, Marcelo D.
Format: Article
Language:English
Subjects:
age
Agronomy. Soil science and plant productions
Amino acids
Biological and medical sciences
cultivo de ovulos
culture d' ovule
Culture media
Developmental biology
Economic plant physiology
edad
embriones vegetales
Embryogenesis
embryon vegetal
Embryos
etapas de desarrollo de la planta
Fruits
Fundamental and applied biological sciences. Psychology
genotipos
genotype
Genotypes
growth rate
In vitro culture
indice de crecimiento
medio de cultivo
milieu de culture
Nitrogen
ovule culture
Ovules
Plant cells
plant developmental stages
plant embryos
Plant physiology and development
Plant reproduction
Plants
stade de developpement vegetal
taux de croissance
Tissue cultures, protoplasts
viabilidad
viabilite
viability
vitis vinifera
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Summary:Embryo enlargement and plant formation via in ovulo embryo culture was studied in stenospermic grapes Genotypes, culture media and their interactions significantly affected embryo enlargement, whereas culture dates did not. Genotype P60-58, when compared to cv Thompson Seedless, had the greatest number of enlarged embryos after 3 months in culture. P60-58 ovules cultured on Cain's basal medium plus L-serine or L-glutamine and cv Thompson Seedless ovules cultured on Cain's basal medium plus L-cysteine or L-asparagine produced the greatest number of enlarged embryos. Viable embryos and morphologically normal-appearing plants were obtained. Polyembryony was observed in cultured zygotic embryos.
ISSN:0002-9122
1537-2197
DOI:10.1002/j.1537-2197.1989.tb11327.x