The effect of bagging on ascorbate in Pyrus fruit

Here, the L-ascorbic acid (AsA) content as well as activities of enzymes and genes in AsA biosynthesis were investigated in bagged and unbagged fruit of Pyrus pyrifolia 'Cuiguan' during on-tree development. The contents of AsA/dehydroascorbate (DHA) and glutathione (γ-glutamyl-L-cysteinylg...

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Bibliographic Details
Published in:New Zealand journal of crop and horticultural science 2019-01, Vol.47 (1), p.19-31
Main Authors: Zhang, Hu Ping, Qin, An, Qi, Kai Jie, Tao, Shu Tian, Wu, Ju You, Huang, Xiao San, Zhang, Shao Ling
Format: Article
Language:English
Subjects:
AsA
ascorbate-glutathione cycle
Ascorbic acid
Bagging
Biosynthesis
Dehydrogenase
Dehydrogenases
Diet
Enzymatic activity
Enzymes
Fruits
Galactose
Gene expression
Genes
Glutathione
Glutathione reductase
L-Ascorbate peroxidase
L-galactose pathway
light avoidance
Metabolism
Peroxidase
Vitamin C
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Summary:Here, the L-ascorbic acid (AsA) content as well as activities of enzymes and genes in AsA biosynthesis were investigated in bagged and unbagged fruit of Pyrus pyrifolia 'Cuiguan' during on-tree development. The contents of AsA/dehydroascorbate (DHA) and glutathione (γ-glutamyl-L-cysteinylglycine, GSH)/oxidised glutathione (GSSG) couples, the enzyme activities of L-galactono-1,4-lactone dehydrogenase (GLDH; EC 1.3.2.3), L-galactose dehydrogenase (GalDH; EC 1.1.1.117), ascorbate peroxidase (APX; EC 1.11.1.11), monodehydroascorbate reductase (MDHAR; EC 1.6.5.4), glutathione-dependent dehydroascorbate reductase (DHAR; EC 1.8.5.1) and glutathione reductase (GR; EC 1.6.4.2), and the relative expression levels of GLDH and DHAR genes were highest at 20 days after full bloom (DAFB) and then decreased to their minimum at harvest. When the fruit were grown in the dark induced by bagging, the ascorbate contents, enzyme activities and gene expression amounts had a statistical decline compared to the control in the most sampling dates. However, although L-AsA metabolism was affected and the use of light transmitting fruit bags may be preferable, the drop in vitamin C was not really significant in a dietary sense.
ISSN:0114-0671
1175-8783
DOI:10.1080/01140671.2018.1462209