In vitro and in vivo antiproliferative activity of organo-nickel SCS-pincer complexes on estrogen responsive MCF7 and MC4L2 breast cancer cells. Effects of amine fragment substitutions on BSA binding and cytotoxicity

A family of organonickel complexes has been prepared, fully characterized, and tested for their antiproliferative activity against estrogen-responsive human breast cancer cells (MCF7). The three SCS-type pincer ligands HL1, HL2, and HL3 and their corresponding Ni(ii) complexes NiL1, NiL2, and NiL3 h...

Full description

Saved in:
Bibliographic Details
Published in:Dalton transactions : an international journal of inorganic chemistry 2018-12, Vol.47 (47), p.16944-16957
Main Authors: Hosseini-Kharat, Mahboubeh, Zargarian, Davit, Alizadeh, Ali Mohammad, Karami, Kazem, Saeidifar, Maryam, Khalighfard, Solmaz, Dubrulle, Laurent, Zakariazadeh, Mostafa, Cloutier, Jean-Philippe, Sohrabijam, Zahra
Format: Article
Language:English
Subjects:
Animals
Anticancer properties
Antineoplastic Agents - chemical synthesis
Antineoplastic Agents - chemistry
Antineoplastic Agents - pharmacology
Antiproliferatives
Binding
Biocompatibility
Breast cancer
Breast Neoplasms - drug therapy
Cancer
Cell Survival - drug effects
Colorimetry
Coordination Complexes - chemical synthesis
Coordination Complexes - chemistry
Coordination Complexes - pharmacology
Crystallography
Cyclohexylamines - chemistry
Data acquisition
Dichroism
Dose-Response Relationship, Drug
Estrogens - chemistry
Female
Fluorescence
Human behavior
Humans
In vivo methods and tests
MCF-7 Cells
Mice, Inbred BALB C
Nickel
Nickel - chemistry
Organometallic Compounds - chemical synthesis
Organometallic Compounds - chemistry
Organometallic Compounds - pharmacology
Proteins
Pyrrolidines - chemistry
Quenching
Serum albumin
Serum Albumin, Bovine - chemistry
Single crystals
Toxicity
Transplantation
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A family of organonickel complexes has been prepared, fully characterized, and tested for their antiproliferative activity against estrogen-responsive human breast cancer cells (MCF7). The three SCS-type pincer ligands HL1, HL2, and HL3 and their corresponding Ni(ii) complexes NiL1, NiL2, and NiL3 have been synthesized and fully characterized, including by single crystal diffraction studies for the complexes. The complexes possess square planar geometry with two symmetrical 5-membered nickellacycles. Fluorescence spectroscopy, circular dichroism measurements, molecular modeling, colorimetric based assay and tumor transplantation studies were used to evaluate the protein binding and antiproliferative activities of these organometallic complexes both in vitro and in vivo. Fluorescence quenching was used to investigate bovine serum albumin (BSA) interaction at different temperatures (293, 303 and 313 K), and the results were analyzed using the classical Stern-Volmer equation, allowing us to propose a dynamic quenching mechanism. Studies in vitro on the antiproliferative activity of the three organonickel complexes against estrogen-responsive human breast cancer cells (MCF7) showed promising antitumor activity for NiL1 containing pyrrolidine fragments. In vivo administration of this compound significantly inhibits tumor growth in estrogen-dependent MC4L2 cancer cells in female BALB/c mice.
ISSN:1477-9226
1477-9234
DOI:10.1039/c8dt03079k