Intracolonic release of nitric oxide during trinitrobenzene sulfonic acid rat colitis

Nitric oxide is thought to play an important role in modulating the inflammatory process. Recently an increase in the inducible form of nitric oxide synthase (iNOS) has been found in the rat trinitrobenzene sulfonic acid model of experimental colitis, and inhibition of nitric oxide synthase activity...

Full description

Saved in:
Bibliographic Details
Published in:Digestive diseases and sciences 1997-12, Vol.42 (12), p.2606-2611
Main Authors: FERRETTI, M, GIONCHETTI, P, RIZZELLO, F, VENTURI, A, STELLA, P, CORTI, F, MIZRAHI, J, MIGLIOLI, M, CAMPIERI, M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Colitis - chemically induced
Colitis - metabolism
Colon - metabolism
Disease Models, Animal
Gastroenterology. Liver. Pancreas. Abdomen
Leukotriene B4 - metabolism
Male
Medical sciences
Nitric Oxide - metabolism
Nitric Oxide Synthase - metabolism
Nitric Oxide Synthase Type II
Nitrites - metabolism
Other diseases. Semiology
Peroxidase - metabolism
Rats
Rats, Sprague-Dawley
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Trinitrobenzenesulfonic Acid
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nitric oxide is thought to play an important role in modulating the inflammatory process. Recently an increase in the inducible form of nitric oxide synthase (iNOS) has been found in the rat trinitrobenzene sulfonic acid model of experimental colitis, and inhibition of nitric oxide synthase activity resulted in an amelioration of tissue injury. The aim of our study was to evaluate in vivo intracolonic release of nitric oxide in this model of colitis. Experimental colitis was induced in male Sprague-Dawley rats by a single intracolonic administration of trinitrobenzene sulfonic acid. Nitrite levels were determined in rectal dialysates by HPLC. The tissue myeloperoxidase and iNOS and the luminal leukotriene B4 were also measured. Nitrite levels were significantly increased in rectal dialysates during colitis and correlated significantly with tissue myeloperoxidase and iNOS activity. The correlation between nitrite dialysate levels and wall iNOS activity confirms that nitrite in dialysates is produced by inflammatory cells and not by colonic bacterial flora. Determination of nitrite levels in rectal dialysates seems a valuable method to monitor colonic inflammation in rat trinitrobenzene sulfonic acid colitis.
ISSN:0163-2116
1573-2568
DOI:10.1023/A:1018897519880