Exploring the gut of Helicoverpa armigera for cellulose degrading bacteria and evaluation of a potential strain for lignocellulosic biomass deconstruction

[Display omitted] •A Klebsiella sp. MD21 strain identified by 16S rRNA gene sequencing was isolated from gut of Helicoverpa armigera.•The isolate demonstrated the utility of lignocellulosic wastes like saw dust, wheat husk etc, as sole source of carbon.•SEM analysis revealed deconstruction of the sa...

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Bibliographic Details
Published in:Process biochemistry (1991) 2018-10, Vol.73, p.142-153
Main Authors: Dar, Mudasir A., Shaikh, Afrin A., Pawar, Kiran D., Pandit, Radhakrishna S.
Format: Article
Language:English
Subjects:
Agro-industrial waste
Bacteria
Biomass
Biorefineries
Cellobiase
Cellulases
Cellulolytic bacteria
Cellulose
Cotton
Diffraction patterns
Dust
Dust filters
Filter paper
Fourier transforms
Gene sequencing
Glucosidase
Helicoverpa armigera
Hydrolysis
Industrial wastes
Insect gut
Klebsiella
Lignocellulose
Microbiota
Molecular sequencing
Pulp
rRNA 16S
Scanning electron microscopy
Sugar
X ray spectra
X-ray diffraction
Xylanase
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Summary:[Display omitted] •A Klebsiella sp. MD21 strain identified by 16S rRNA gene sequencing was isolated from gut of Helicoverpa armigera.•The isolate demonstrated the utility of lignocellulosic wastes like saw dust, wheat husk etc, as sole source of carbon.•SEM analysis revealed deconstruction of the saw dust and filter paper after treatment with Klebsiella sp. MD21.•The study validates the insect gut as a source for isolation of cellulose degrading bacteria. The present study demonstrates the enrichment, isolation and identification of cellulose degrading bacteria from the gut of cotton bollworm, Helicoverpa armigera. The isolate MD21 was identified as Klebsiella sp. MD21 (MG367463) based on 16S rRNA gene sequencing. The isolated bacterium, Klebsiella sp. MD21 secreted many hydrolytic enzymes particularly xylanase and β-glucosidase with highest activities of 276.71 ± 0.98 and 78.45 ± 0.10 IU/ml extract respectively. Scanning electron microscopy of treated filter paper and saw dust revealed structural alterations caused by Klebsiella sp. MD21 leading to hydrolysis of cellulosic waste into reducing sugars (2256 μmol ml−1  min-1) which could be used for the growth of bacteria. X-ray diffraction (XRD) pattern showed an increase in crystallinity index from 58.78% in native biomass to 60.10% of the treated biomass due to hydrolysis of amorphous regions present in the saw dust. Similarly, fourier transform infra-red (FTIR) spectroscopic study of the hydrolyzed saw dust indicated elimination of bands at wave numbers 718, 933 and 1533 cm-1 which also represent the cellulose content. The present study demonstrates utility of H. armigera as a source for isolation of cellulolytic bacteria for use in biorefinery and pulp industries.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2018.08.001