The capsid assembly-induced luminescence enhancement (AILE) of DNA-protected silver nanoclusters and an in situ application

Two assembly modes have been constructed between double-strand DNA-protected silver nanoclusters (AgNCs-dsDNA) and human papillomavirus (HPV) 16 main capsid protein L1, respectively, which are well defined by CsCl gradient centrifugation, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SD...

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Bibliographic Details
Published in:New journal of chemistry 2018, Vol.42 (21), p.17492-17498
Main Authors: Jia, Xiang-Yu, Xue, Ya-Rong, Li, Hong-Wei, Fu, Ding-Yi, Wang, Wei-Xian, Wu, Yuqing
Format: Article
Language:English
Subjects:
Assembly
Deoxyribonucleic acid
Dismantling
DNA
Electrophoresis
Elution
Fluorescence
Human papillomavirus
Luminescence
Monitoring
Nanoclusters
Photon correlation spectroscopy
Proteins
Silver
Sodium dodecyl sulfate
Transmission electron microscopy
Viruses
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Summary:Two assembly modes have been constructed between double-strand DNA-protected silver nanoclusters (AgNCs-dsDNA) and human papillomavirus (HPV) 16 main capsid protein L1, respectively, which are well defined by CsCl gradient centrifugation, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), dynamic light scattering (DLS) and transmission electron microscopy (TEM). The co-assembly leads to a large emission increase of AgNCs-dsDNA attributed to an assembly-induced luminescence enhancement (AILE), and vice versa in disassembly; however, the post-assembly does not. Therefore, a new approach to monitoring the assembly/disassembly of HPV virus-like particles (VLPs) is established accordingly. The study not only presents an AILE phenomenon for silver nanoclusters but also affords an easily performed fluorescence approach to evaluate HPV VLP assembly/disassembly directly, which could be extended to other kinds of metal nanoclusters protected by DNA as well as a kinetic study on the assembly of other species of virus.
ISSN:1144-0546
1369-9261
DOI:10.1039/C8NJ03179G