Enzyme-powered motility in buoyant organoclay/DNA protocells

Reconstitution and simulation of cellular motility in microcompartmentalized colloidal objects have important implications for microcapsule-based remote sensing, environmentally induced signalling between artificial cell-like entities and programming spatial migration in synthetic protocell consorti...

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Bibliographic Details
Published in:Nature chemistry 2018-11, Vol.10 (11), p.1154-1163
Main Authors: Kumar, B V V S Pavan, Patil, Avinash J, Mann, Stephen
Format: Article
Language:English
Subjects:
Biocatalysis
Bubbles
Buoyancy
Capsules
Catalase
Catalase - chemistry
Clay - chemistry
Colloids
Colloids - chemistry
Consortia
Deoxyribonucleic acid
DNA
DNA - chemistry
Enzymes
Enzymes - chemistry
Flotation
Glucose oxidase
Glucose Oxidase - chemistry
Hydrogen peroxide
Hydrogen Peroxide - chemistry
Microcapsules
Microencapsulation
Motility
Object recognition
Organic chemistry
Remote monitoring
Remote sensing
Vertical motion
Vertical oscillations
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Summary:Reconstitution and simulation of cellular motility in microcompartmentalized colloidal objects have important implications for microcapsule-based remote sensing, environmentally induced signalling between artificial cell-like entities and programming spatial migration in synthetic protocell consortia. Here we describe the design and construction of catalase-containing organoclay/DNA semipermeable microcapsules, which in the presence of hydrogen peroxide exhibit enzyme-powered oxygen gas bubble-dependent buoyancy. We determine the optimum conditions for single and/or multiple bubble generation per microcapsule, monitor the protocell velocities and resilience, and use remote magnetic guidance to establish reversible changes in the buoyancy. Co-encapsulation of catalase and glucose oxidase is exploited to establish a spatiotemporal response to antagonistic bubble generation and depletion to produce protocells capable of sustained oscillatory vertical movement. We demonstrate that the motility of the microcapsules can be used for the flotation of macroscopic objects, self-sorting of mixed protocell communities and the delivery of a biocatalyst from an inert to chemically active environment. These results highlight new opportunities to constructing programmable microcompartmentalized colloids with buoyancy-derived motility.
ISSN:1755-4330
1755-4349
DOI:10.1038/s41557-018-0119-3