Embryology. Comparison of in-vitro development of embryos originating from either conventional in-vitro fertilization or intracytoplasmic sperm injection

In this retrospective study on 1628 consecutive cycles performed during a period of 4 years, development in vitro is compared of embryos obtained after either conventional in-vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). At 39-42 h after insemination or injection, embryos obt...

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Published in:Human reproduction (Oxford) 2000-02, Vol.15 (2), p.402
Main Authors: John C.M. Dumoulin, Coonen, Edith, Bras, Marijke, Lucie C.P. van Wissen, Ignoul-Vanvuchelen, Rosie, J.Marij Bergers-Jansen, Derhaag, Josien G, Joep P.M. Geraedts, Johannes L.H. Evers
Format: Article
Language:English
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Summary:In this retrospective study on 1628 consecutive cycles performed during a period of 4 years, development in vitro is compared of embryos obtained after either conventional in-vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). At 39-42 h after insemination or injection, embryos obtained after ICSI were significantly (P < 0.01) further developed (mean cell number 3.48 + or - 0.03) as compared with those obtained after IVF (3.22 + or - 0.03), whereas after 63-66 h of in-vitro development this difference was no longer present (mean cell number 6.11 + or - 0.15 versus 6.09 + or - 0.13 respectively). Culture of surplus embryos obtained after IVF resulted in a significantly higher (P < 0.001) mean incidence of blastocyst formation per cycle as compared with the ICSI group (31.8 + or - 1.9 versus 23.0 + or - 1.4 respectively). Blastocysts from both groups consisted of comparable numbers of cells. Blastocyst formation was also significantly higher when embryos were cultured in groups (31.2 + or - 1.8) compared to single culture (23.1 + or - 1.5; P < 0.01), in human tubal fluid (HTF) medium (29.2 + or - 1.7) compared with IVF-50
ISSN:0268-1161
1460-2350