Effects of Chronic Exposure to Ultraviolet B Radiation on DNA Repair in the Dermis and Epidermis of the Hairless Mouse

It has previously been shown that chronic exposure to low fluences of ultraviolet B radiation reduced DNA repair capacity in mouse skin. In this study we now extend this to examine the concentration dependence and tissue dependence of this phenomenon. We found that (6–4) photoproducts were repaired...

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Bibliographic Details
Published in:Journal of investigative dermatology 2001-02, Vol.116 (2), p.209-215
Main Authors: Mitchell, David L., Byrom, Michelle, Chiarello, Stephanie, Lowery, Megan G.
Format: Article
Language:English
Subjects:
(6–4) photoproduct
Animals
Biological and medical sciences
cyclobutane dimer
DNA Repair - radiation effects
Dose-Response Relationship, Radiation
Female
Injuries of the skin. Diseases of the skin due to physical agents
Medical sciences
Mice
Mice, Hairless
nucleotide excision repair
Skh-1 mouse
Skin - radiation effects
Traumas. Diseases due to physical agents
Ultraviolet Rays
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Summary:It has previously been shown that chronic exposure to low fluences of ultraviolet B radiation reduced DNA repair capacity in mouse skin. In this study we now extend this to examine the concentration dependence and tissue dependence of this phenomenon. We found that (6–4) photoproducts were repaired considerably faster than cyclobutane dimers and that the kinetics for photoproduct removal were comparable in the dermis and epidermis. Chronic ultraviolet B irradiation significantly reduced the initial rate and extent of DNA repair. After low daily doses of ultraviolet B (6–4) photoproduct repair was most affected and after high daily doses the repair of both cyclobutane and (6–4) dimers was reduced. Whereas cyclobutane dimer repair was most affected in the dermis, reduced (6–4) photoproduct repair was observed in both tissues. The deleterious effects of chronic ultraviolet exposure were sustained for a considerable time after the chronic treatment ended.
ISSN:0022-202X
1523-1747
DOI:10.1046/j.1523-1747.2001.01192.x