The sequence analysis of Epstein–Barr virus EBNA1 gene: could viral screening markers for nasopharyngeal carcinoma be identified?

Epstein–Barr virus (EBV) has been identified as a group 1 carcinogenic agent, particularly for nasopharyngeal carcinoma (NPC). The sequence diversity of EBV nuclear antigen 1 (EBNA1) reflects region-restricted polymorphisms, which may be associated with the development of certain malignancies. The a...

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Bibliographic Details
Published in:Medical microbiology and immunology 2019-02, Vol.208 (1), p.81-88
Main Authors: Banko, Ana V., Lazarevic, Ivana B., Karalic, Danijela Z., Djukic, Vojko B., Cupic, Maja D., Stevanovic, Goran, Jovanovic, Tanja P.
Format: Article
Language:English
Subjects:
Bioindicators
Biomedical and Life Sciences
Biomedicine
Epstein-Barr virus
Immunology
Infectious mononucleosis
Kidney transplantation
Medical Microbiology
Mononucleosis
Nasopharyngeal carcinoma
Organs
Original Investigation
Phylogeny
Samples
Statistical analysis
Throat cancer
Transplantation
Virology
Viruses
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Summary:Epstein–Barr virus (EBV) has been identified as a group 1 carcinogenic agent, particularly for nasopharyngeal carcinoma (NPC). The sequence diversity of EBV nuclear antigen 1 (EBNA1) reflects region-restricted polymorphisms, which may be associated with the development of certain malignancies. The aims of the present study were to evaluate EBV EBNA1 gene polymorphisms circulating in NPC, infectious mononucleosis, and isolates from patients with transplanted organs to determine if EBNA1 sequence specificities are useful as viral biomarkers for NPC. Forty biopsies of undifferentiated carcinoma of nasopharyngeal type (UCNT), 31 plasma samples from patients with mononucleosis syndrome, and 16 plasma samples from patients after renal transplantation were tested in this study. The EBNA1 gene was amplified by nested PCR. Further investigation included sequencing, phylogenetic, and statistical evaluations. Eighty-seven sequences were identified as one of the four EBNA1 subtypes, P-Ala, P-Thr, V-Val, and V-Ala, with further classification into ten subvariants. Of these, P-Thr-sv-1 and P-Thr-sv-3 have never been identified in Europe, while V-Val-sv-1 was newly discovered. Statistical analysis revealed significant differences in the distribution of EBNA1 P-Thr subvariants between the three groups of patients, with noticeable clustering of P-Thr-sv-5 in NPC isolates ( p  
ISSN:0300-8584
1432-1831
DOI:10.1007/s00430-018-0561-2