Removal of phenolic compounds through overliming for enhanced saccharification of wheat straw

BACKGROUND The deactivation of cellulases by phenolics is considered to be the main bottleneck in the saccharification of agricultural by‐products for efficient bioethanol production on a large scale. The removal of these phenolic inhibitors, produced as a result of pretreatment, is essential for an...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chemical technology and biotechnology (1986) 2018-10, Vol.93 (10), p.3011-3017
Main Authors: Haq, Ikram, Arshad, Yesra, Nawaz, Ali, Aftab, Muhammad, Rehman, Asad, Mukhtar, Hamid, Mansoor, Zinnia, Syed, Qurratulain
Format: Article
Language:English
Subjects:
Alkalies
Alkalis
Ammonium hydroxide
bioconversion
Biofuels
Biomass
Calcium hydroxide
catalysis
Deactivation
Detoxification
Ethanol
Gallic acid
Hydrolysates
Organic chemistry
Phenolic compounds
Phenols
Pretreatment
process optimization
Saccharification
Scanning electron microscopy
Slaked lime
Sodium hydroxide
Straw
Substrates
Weight
Wheat
Wheat straw
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:BACKGROUND The deactivation of cellulases by phenolics is considered to be the main bottleneck in the saccharification of agricultural by‐products for efficient bioethanol production on a large scale. The removal of these phenolic inhibitors, produced as a result of pretreatment, is essential for an efficient saccharification process. One such process is overliming that has been mostly studied for hydrolysates of agricultural by‐products. In the present study however, solid biomass was used whereby the detection of phenolic compounds and their effect on saccharification was studied. RESULTS The pretreated wheat straw showed 19% saccharification and total phenolic content (TPC) of 188 mg gallic acid equivalent g‐1 biomass dry weight. Detoxification of pretreated wheat straw applying different alkalis, i.e. NaOH, NH4OH and Ca(OH)2 was conducted. Overliming using Ca(OH)2 solution of pH 12, for 2 h at 80°C showed significant removal of 60% of phenolic compounds, which in turn enhanced the saccharification by 2.4‐fold. Scanning electron microscopy of pretreated and overlimed substrates also showed considerable change in structure, making it more accessible for enzymatic hydrolysis. CONCLUSION The elimination of phenolic compounds due to overliming has facilitated increased saccharification that in turn will help in substrate efficient biofuel production. © 2018 Society of Chemical Industry
ISSN:0268-2575
1097-4660
DOI:10.1002/jctb.5659