Degradation of atrazine by Pseudomonas sp. and Achromobacter sp. isolated from Brazilian agricultural soil

Atrazine is a herbicide used worldwide to control weeds in maize, sorghum and sugarcane crops. This study presents two bacteria isolated from Brazilian soil samples with atrazine application history. The isolates, identified as Pseudomonas sp. and Achromobacter sp., showed potential to degrade atraz...

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Bibliographic Details
Published in:International biodeterioration & biodegradation 2018-05, Vol.130, p.17-22
Main Authors: Tonelli Fernandes, Ana Flavia, Braz, Vânia Santos, Bauermeister, Anelize, Rizzato Paschoal, Jonas Augusto, Lopes, Norberto Peporine, Stehling, Eliana Guedes
Format: Article
Language:English
Subjects:
Achromobacter
Agricultural land
Agrochemicals
Atrazine
atz genes
Biodegradation
Cereal crops
Corn
Degradation
Gene expression
Genes
Herbicides
HPLC
Incubation
Metabolites
Pseudomonas
Soil bacteria
Sorghum
Sugarcane
Weed control
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Summary:Atrazine is a herbicide used worldwide to control weeds in maize, sorghum and sugarcane crops. This study presents two bacteria isolated from Brazilian soil samples with atrazine application history. The isolates, identified as Pseudomonas sp. and Achromobacter sp., showed potential to degrade atrazine in solid medium. Pseudomonas sp. exhibited all the six atz genes that encode enzymes of the degradation process. This isolate was capable of degrading 99% of atrazine in vitro after 24 h of incubation. Achromobacter sp. presented only atzA, atzB and atzC genes, which are responsible for the first steps of the degradation pathway, and showed a different degradation profile. The three initial metabolites formed by atrazine degradation were detected in samples containing both Pseudomonas sp. and Achromobacter sp., suggesting atrazine transformation. By Northern Blot assay, atzA, atzB, atzC and atzD genes were differentially expressed in the presence of atrazine in Pseudomonas sp., however, this difference was not observed in Achromobacter sp. This study confirms the worldwide dispersion of atz genes. [Display omitted] •Pseudomonas sp. A02 degraded 99% of atrazine in vitro after 24 h.•Achromobacter sp. A01 partially degraded 39% of atrazine in vitro after 48 h.•The consortium formed by A01 and A02 was not more efficient to degrade atrazine.•Genes atz genes had differential expression in isolate A02.
ISSN:0964-8305
1879-0208
DOI:10.1016/j.ibiod.2018.03.011