Effects of chitosan-coated lipid-core nanocapsules on bovine sperm cells

Toxicology studies have a pivotal role for selection of new nanosystems. As lipid-core nanocapsules (LNC) rise as a potential system not only for drug delivery but also for immunotherapy and gene therapy, the demand for models of toxic screening increases, and sperm arises as a promising model due t...

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Bibliographic Details
Published in:Toxicology in vitro 2017-04, Vol.40, p.214-222
Main Authors: de Castro Jorge Silva, Arthur, Remirão, Mariana Härter, Lucas, Caroline Gomes, Domingues, William Borges, Silveira, Tony, Paschoal, Julia Dame, Jornada, Denise S., Corcine, Carine Dahl, Junior, Antonio Sergio Varela, Prado, Willian A., Campos, Vinicius Farias, Seixas, Fabiana Kömmling, Guterres, Silvia S., Pohlmann, Adriana R., Collares, Tiago
Format: Article
Language:English
Subjects:
Animals
Cattle
Chitosan
Chitosan - chemistry
Chitosan - toxicity
Coating effects
Coatings
DNA Damage
Drug delivery
Drug delivery systems
Flow cytometry
Gene therapy
Immunotherapy
Lipid Peroxidation - drug effects
Lipids
Lipids - chemistry
Lipids - toxicity
Male
Mathematical models
Membrane Potential, Mitochondrial - drug effects
Nanocapsules
Nanocapsules - chemistry
Nanocapsules - toxicity
Nanosystem
Parameters
Sperm
Sperm Motility - drug effects
Spermatozoa - drug effects
Spermatozoa - metabolism
Spermatozoa - physiology
Spermtoxicology
Toxicity
Toxicology
Viability
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Summary:Toxicology studies have a pivotal role for selection of new nanosystems. As lipid-core nanocapsules (LNC) rise as a potential system not only for drug delivery but also for immunotherapy and gene therapy, the demand for models of toxic screening increases, and sperm arises as a promising model due to the easiness to evaluate its viability parameters. LNCs were coated with chitosan, chitosan-coated lipid-core nanocapsules (LNC-CS), in order to modify the nanocapsule surface. We evaluated the toxicity of LNC and LNC-CS after incubation with bovine sperm in different concentrations (2.5%, 5%, 10%, 20%, 40% and 80%) (v/v) and periods of exposure (0h and 1h). CASA parameters and flow cytometry assays were performed to assess LNC and LNC-CS effects. The results corroborated with previous studies indicating that there is no toxicity from LNCs and LNC-CS below 40% (v/v) concentration. •Lipid-core nanocapsules (LNC) were coated with chitosan (CS).•Toxicity of LNC and LNC-CS after incubation with bovine sperm in different concentrations•CASA parameters and flow cytometry assays were performed to assess LNC and LNC-CS effects.•There is no toxicity from LNCs and LNC-CS above 40% (v/v) concentration.
ISSN:0887-2333
1879-3177
DOI:10.1016/j.tiv.2017.01.017