Beneficial effects of Caesalpinia digyna extract against acid aspiration-Induced acute lung injury in mice

Objective: Caesalpinia digyna belongs to the genus Caesalpinia, which is known since ancient times for its medicinal properties. The present work was designed to evaluate the beneficial potential of hydroalcoholic extract of the roots of C. dignea against hydrochloric acid (HCl)-induced acute lung i...

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Published in:Pharmacognosy research 2018-07, Vol.10 (3), p.243-249
Main Authors: Chaudhary, Manju, Sharma, Pratibha, Mittal, Meenu, Kaur, Rajneet, Dharwal, Vivek, Kumar, Ashwani, Naura, Amarjit
Format: Article
Language:English
Subjects:
Analysis
Antioxidants
Biochemistry
Chromatography
Cytokines
Hydrochloric acid
Inflammation
Lungs
Neutrophils
NMR
Nuclear magnetic resonance
Oxidative stress
Pathogenesis
Reagents
Respiratory distress syndrome
Rodents
Sepsis
Trauma
Tumor necrosis factor-TNF
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Summary:Objective: Caesalpinia digyna belongs to the genus Caesalpinia, which is known since ancient times for its medicinal properties. The present work was designed to evaluate the beneficial potential of hydroalcoholic extract of the roots of C. dignea against hydrochloric acid (HCl)-induced acute lung injury in mice. Materials and Methods: Ethanolic extract of C. dignea roots at a dose of 50, 100, or 200 mg/kg boy weight was given once orally 90 min before HCl administration. Mice were then analyzed for infiltration of inflammatory cells in bronchoalveolar lavage fluid (BALF) and oxidative stress markers in the lung tissue. Further, the effects of the extract were compared with bergenin isolated from the extract. Results: Our results showed that an oral administration of the extract 90 min before HCl instillation reduced the infiltration of neutrophils in the lungs in a dose-dependent manner. Reduction in lung inflammation was associated with decline in pulmonary edema as the total protein content in the BALF was found to be decreased substantially. The drug also restored the redox balance in the lungs toward normal on HCl treatment as assessed by measuring the levels of reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), and catalase activity. Bergenin, isolated from the plant, was able to suppress the neutrophils but increased the macrophage number in BALF when administered before HCl instillation, suggesting immunoregulatory properties of the key constituent of the extract. Conclusion: Our data suggest that hydroalcoholic extract of Caesalpinia digyna roots constitute the phytochemicals that can protect against HCl-induced acute lung injury in mice. Abbreviations Used: ALI: Acute lung injury, BALF: Bronchoalveolar lavage fluid, MDA: Malondialdehyde, ROS: Reactive oxygen species, DCF-DA: 2' 7'-dichlorofluorescein diacetate
ISSN:0974-8490
0976-4836
0974-8490
DOI:10.4103/pr.pr_4_18