Label-free gold nanoparticle-based sensing of cysteine: New peculiarities and prospects

Label-free gold nanoparticle-based sensing of cysteine: New peculiarities and prospects

[Display omitted] •AuNPs aggregation behaviors in solution and on polyurethane surface are different.•EDTA is recommended for both AuNPs destabilization and elimination of interferences.•Interaction of AuNPs on polyurethane foam with cysteine doesn’t require any destabilization.•Sensing of cysteine...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2018-05, Vol.260, p.953-961
Main Authors: Apyari, Vladimir V., Arkhipova, Viktoria V., Isachenko, Andrei I., Volkov, Pavel A., Dmitrienko, Stanislava G., Torocheshnikova, Irina I.
Format: Article
Language:eng
Subjects:
Agglomeration
Amino acids
Cysteamine
Cysteine
Diffuse reflectance spectroscopy
Ethylenediaminetetraacetic acids
Gold
Label-free detection
Label-free gold nanoparticles
Metals
Nanoparticle aggregation
Nanoparticles
Polyurethane foam
Reflectance
Sensitivity
Sensors
Spectrophotometry
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Summary:[Display omitted] •AuNPs aggregation behaviors in solution and on polyurethane surface are different.•EDTA is recommended for both AuNPs destabilization and elimination of interferences.•Interaction of AuNPs on polyurethane foam with cysteine doesn’t require any destabilization.•Sensing of cysteine using spectrophotometry or diffuse reflection is described. In this article, some new peculiarities and prospects of cysteine determination using aggregation of label-free gold nanoparticles (AuNPs) are described. Features of the aggregation both in solution and on polyurethane surface are compared. Effects of ethylenediaminetetraacetate (EDTA) and cysteamine on the aggregation of AuNPs, which increase sensitivity, are considered. In case of EDTA, it was simultaneously possible to eliminate interferences from cations of metals. It is shown that attaching AuNPs onto polyurethane foam surface changes their sensitivity and selectivity, which can be used for the determination of cysteine. Based on found peculiarities, new methods for the determination of cysteine in a range 0.06–0.5 μg mL−1 (LOD = 0.04 μg mL−1) by spectrophotometry, and 0.02–0.15 μg mL−1 (LOD = 0.01 μg mL−1) by diffuse reflectance spectroscopy were developed.
ISSN:0925-4005
1873-3077