Inactivation of Adenoviruses, Enteroviruses, and Murine Norovirus in Water by Free Chlorine and Monochloramine

Inactivation of infectious viruses during drinking water treatment is usually achieved with free chlorine. Many drinking water utilities in the United States now use monochloramine as a secondary disinfectant to minimize disinfectant by-product formation and biofilm growth. The inactivation of human...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2010-02, Vol.76 (4), p.1028-1033
Main Authors: Cromeans, Theresa L, Kahler, Amy M, Hill, Vincent R
Format: Article
Language:English
Subjects:
Adenoviridae
Animals
Biological and medical sciences
Chloramines - pharmacology
Chlorine
Chlorine - pharmacology
Disinfectants - pharmacology
Disinfection - methods
Drinking water
Enterovirus - drug effects
Fresh Water - virology
Fundamental and applied biological sciences. Psychology
Health risk assessment
Humans
Mice
Microbiology
Norovirus
Norovirus - drug effects
Public Health Microbiology
United States
Virus Inactivation - drug effects
Viruses
Water Purification - methods
Water Supply - standards
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Summary:Inactivation of infectious viruses during drinking water treatment is usually achieved with free chlorine. Many drinking water utilities in the United States now use monochloramine as a secondary disinfectant to minimize disinfectant by-product formation and biofilm growth. The inactivation of human adenoviruses 2, 40, and 41 (HAdV2, HAdV40, and HAdV41), coxsackieviruses B3 and B5 (CVB3 and CVB5), echoviruses 1 and 11 (E1 and E11), and murine norovirus (MNV) are compared in this study. Experiments were performed with 0.2 mg of free chlorine or 1 mg of monochloramine/liter at pH 7 and 8 in buffered reagent-grade water at 5°C. CT values (disinfectant concentration x time) for 2- to 4-log₁₀ (99 to 99.99%) reductions in virus titers were calculated by using the efficiency factor Hom model. The enteroviruses required the longest times for chlorine inactivation and MNV the least time. CVB5 required the longest exposure time, with CT values of 7.4 and 10 mg·min/liter (pH 7 and 8) for 4-log₁₀ inactivation. Monochloramine disinfection was most effective for E1 (CT values ranged from 8 to 18 mg·min/liter for 2- and 3-log₁₀ reductions, respectively). E11 and HAdV2 were the least susceptible to monochloramine disinfection (CT values of 1,300 and 1,600 mg-min/liter for 3-log₁₀ reductions, respectively). Monochloramine inactivation was most successful for the adenoviruses, CVB5, and E1 at pH 7. A greater variation in inactivation rates between viruses was observed during monochloramine disinfection than during chlorine disinfection. These data will be useful in drinking water risk assessment studies and disinfection system planning.
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/aem.01342-09