Transformation of folate-consuming Lactobacillus gasseri into a folate producer

Five genes essential for folate biosynthesis in Lactococcus lactis were cloned on a broad-host-range lactococcal vector and were transferred to the folate auxotroph Lactobacillus gasseri. As a result L. gasseri changed from a folate consumer to a folate producer. This principle can be used to increa...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2004-05, Vol.70 (5), p.3146-3148
Main Authors: Wegkamp, A, Starrenburg, M, Vos, W.M. de, Hugenholtz, J, Sybesma, W
Format: Article
Language:English
Subjects:
auxotrophs
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biological and medical sciences
biosynthesis
Biotechnology - methods
Cloning
Cloning, Molecular
DNA, Bacterial - genetics
expression
fermented foods
folate biosynthetic genes
folic acid
Folic Acid - biosynthesis
Folic Acid - metabolism
Food Microbiology
Food science
Fundamental and applied biological sciences. Psychology
gene
Gene Expression Regulation, Bacterial
gene transfer
Genes
genetic transformation
lactic-acid bacteria
Lactobacillus - genetics
Lactobacillus - metabolism
Lactobacillus gasseri
Lactococcus lactis
Lactococcus lactis - genetics
Lactococcus lactis - metabolism
Microbiology
molecular cloning
Multigene Family
plasmid vectors
Plasmids
starter cultures
structural genes
Transformation, Bacterial
Vitamin B
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Summary:Five genes essential for folate biosynthesis in Lactococcus lactis were cloned on a broad-host-range lactococcal vector and were transferred to the folate auxotroph Lactobacillus gasseri. As a result L. gasseri changed from a folate consumer to a folate producer. This principle can be used to increase folate levels in many fermented food products.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.70.5.3146-3148.2004