Rapid Method for Enumeration of Viable Legionella pneumophila and Other Legionella spp. in Water

A sensitive and specific method has been developed to enumerate viable L. pneumophila and other Legionella spp. in water by epifluorescence microscopy in a short period of time (a few hours). This method allows the quantification of L. pneumophila or other Legionella spp. as well as the discriminati...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2005-07, Vol.71 (7), p.4086-4096
Main Authors: Delgado-Viscogliosi, Pilar, Simonart, Tristan, Parent, Virginie, Marchand, Grégory, Dobbelaere, Marie, Pierlot, Eric, Pierzo, Véronique, Menard-Szczebara, Florence, Gaudard-Ferveur, Elisabeth, Delabre, Karine, Delattre, Jean Marie
Format: Article
Language:English
Subjects:
Antibodies
Antibodies, Bacterial - immunology
Bacteria
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Colony Count, Microbial
Culture Media
Disinfection - methods
Fluorescent Antibody Technique
Fresh Water - microbiology
Fundamental and applied biological sciences. Psychology
Groundwater
Legionella
Legionella - growth & development
Legionella - isolation & purification
Legionella pneumophila - growth & development
Legionella pneumophila - isolation & purification
Methods
Microbiology
Microscopy, Fluorescence - methods
Reproducibility of Results
Sensitivity and Specificity
Species Specificity
Staining and Labeling
Time Factors
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Summary:A sensitive and specific method has been developed to enumerate viable L. pneumophila and other Legionella spp. in water by epifluorescence microscopy in a short period of time (a few hours). This method allows the quantification of L. pneumophila or other Legionella spp. as well as the discrimination between viable and nonviable LEGIONELLA: It simultaneously combines the specific detection of Legionella cells using antibodies and a bacterial viability marker (ChemChrome V6), the enumeration being achieved by epifluorescence microscopy. The performance of this immunological double-staining (IDS) method was investigated in 38 natural filterable water samples from different aquatic sources, and the viable Legionella counts were compared with those obtained by the standard culture method. The recovery rate of the IDS method is similar to, or higher than, that of the conventional culture method. Under our experimental conditions, the limit of detection of the IDS method was
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.71.7.4086-4096.2005