Pharmacologic overview of systemic chlorogenic acid therapy on experimental wound healing

Chlorogenic acid (CGA) is a well-known natural antioxidant in human diet. To understand the effects of CGA on wound healing by enhancing antioxidant defense in the body, the present study sought to investigate the potential role of systemic CGA therapy on wound healing and oxidative stress markers o...

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Published in:Naunyn-Schmiedeberg's archives of pharmacology 2014-11, Vol.387 (11), p.1101-1116
Main Authors: Bagdas, Deniz, Gul, Nihal Yasar, Topal, Ayse, Tas, Sibel, Ozyigit, Musa Ozgur, Cinkilic, Nilufer, Gul, Zulfiye, Etoz, Betul Cam, Ziyanok, Sedef, Inan, Sevda, Turacozen, Ozge, Gurun, Mine Sibel
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Anti-Inflammatory Agents - administration & dosage
Anti-Inflammatory Agents - pharmacology
Anti-Inflammatory Agents - toxicity
Antioxidants
Antioxidants - administration & dosage
Antioxidants - pharmacology
Antioxidants - toxicity
Biomedical and Life Sciences
Biomedicine
Bone marrow
Catalase
Chlorogenic acid
Chlorogenic Acid - administration & dosage
Chlorogenic Acid - pharmacology
Chlorogenic Acid - toxicity
Collagen
Cytotoxicity
Dietary supplements
Dose-Response Relationship, Drug
Free Radical Scavengers - administration & dosage
Free Radical Scavengers - pharmacology
Free Radical Scavengers - toxicity
Glutathione
Hydroxyproline
Inflammation
Kidneys
Leukocytes
Light effects
Lipid peroxidation
Lipid Peroxidation - drug effects
Liver
Male
Malondialdehyde
Matrix metalloproteinase
Metalloproteinase
Micronuclei
Muscles
Neurosciences
Nitric oxide
Nitric Oxide - metabolism
Organs
Original Article
Oxidative stress
Oxidative Stress - drug effects
Peroxidation
Pharmacology/Toxicology
Rats
Rats, Sprague-Dawley
Rodents
Side effects
Skin
Skin - drug effects
Skin - metabolism
Superoxide dismutase
Wound healing
Wound Healing - drug effects
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Summary:Chlorogenic acid (CGA) is a well-known natural antioxidant in human diet. To understand the effects of CGA on wound healing by enhancing antioxidant defense in the body, the present study sought to investigate the potential role of systemic CGA therapy on wound healing and oxidative stress markers of the skin. We also aimed to understand whether chronic CGA treatment has side effects on pivotal organs or rat bone marrow during therapy. Full-thickness experimental wounds were created on the backs of rats. CGA (25, 50, 100, 200 mg/kg) or vehicle was administered intraperitoneally for 15 days. All rats were sacrificed on the 16th day. Biochemical, histopathological, and immunohistochemical examinations were performed. Possible side effects were also investigated. The results suggested that CGA accelerated wound healing in a dose-dependent manner. CGA enhanced hydroxyproline content, decreased malondialdehyde and nitric oxide levels. and elevated reduced glutathione, superoxide dismutase, and catalase levels in wound tissues. Epithelialization, angiogenesis, fibroblast proliferation, and collagen formation increased by CGA while polymorph nuclear leukocytes infiltration decreased. CGA modulated matrix metalloproteinase-9 and tissue inhibitor-2 expression in biopsies. Otherwise, high dose of CGA increased lipid peroxidation of liver and kidney without affecting the heart and muscle samples. Chronic CGA increased micronuclei formation and induced cytotoxicity in the bone marrow. In conclusion, systemic CGA has beneficial effects in improving wound repair. Antioxidant, free radical scavenger, angiogenesis, and anti-inflammatory effects of CGA may ameliorate wound healing. High dose of CGA may induce side effects. In light of these observations, CGA supplementation or dietary CGA may have benefit on wound healing. Graphical abstract ᅟ
ISSN:0028-1298
1432-1912
DOI:10.1007/s00210-014-1034-9