Cytokine Concentrations in Bone Marrow of Stable Sickle Cell Anemia Patients

Inflammation plays a significant role in the clinical manifestations of sickle cell anemia. In studies of anemic patients with other clinical syndromes, measurement of the concentrations of cytokine mediators of inflammation in bone marrow aspirates has provided unique correlations with clinical and...

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Bibliographic Details
Published in:Journal of investigative medicine 2007-03, Vol.55 (2), p.69-74
Main Authors: Dallalio, Gail, Brunson, Chris Y., Means, Robert T.
Format: Article
Language:English
Subjects:
Anemia, Sickle Cell - metabolism
Apoptosis
Biomarkers - analysis
Blood
Bone marrow
Bone Marrow - metabolism
Case-Control Studies
Chronic illnesses
Cytokines
Cytokines - analysis
Cytokines - metabolism
Erythropoiesis
Growth factors
Humans
Inflammation
Interleukin-1 - analysis
Interleukin-1 - metabolism
Interleukin-6 - analysis
Interleukin-6 - metabolism
Placenta
Placenta Growth Factor
Pregnancy Proteins - analysis
Pregnancy Proteins - metabolism
R&D
Receptors, Transferrin - analysis
Receptors, Transferrin - metabolism
Research & development
Review boards
Rheumatoid arthritis
Sickle cell anemia
Sickle cell disease
Tumor necrosis factor-TNF
Tumor Necrosis Factors - analysis
Tumor Necrosis Factors - metabolism
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Summary:Inflammation plays a significant role in the clinical manifestations of sickle cell anemia. In studies of anemic patients with other clinical syndromes, measurement of the concentrations of cytokine mediators of inflammation in bone marrow aspirates has provided unique correlations with clinical and laboratory parameters. We determined concentrations of interleukin (IL)-1, IL-6, tumor necrosis factor (TNF), and placental growth factor (PlGF) in bone marrow aspirates from six homozygous sickle cell (SS) patients who were not acutely ill and who were not receiving hydroxyurea, erythropoietin, or chronic transfusion and compared them with specimens from seven healthy controls. We also measured concentrations of soluble transferrin receptor (sTfR) and of marrow erythroid colony-forming units (CFU-E) as markers of erythropoietic activity. sTfR concentration was significantly higher in SS patients (p = .024). CFU-E concentration was not significantly different between the two groups. Bone marrow concentrations of IL-6 and IL-1 did not differ between the study groups. TNF was undetectable in all specimens, plasma or marrow. Bone marrow PlGF concentrations were significantly higher in SS patients (p = .004). Since PlGF is a product of erythroid cells, the ratio of marrow PlGF to marrow sTfR was determined and found to be significantly greater in SS patients. This suggests that the observed difference in marrow PlGF concentrations does not reflect increased erythropoiesis but rather represents increased PlGF production per erythroid unit.
ISSN:1081-5589
1708-8267
DOI:10.2310/6650.2007.06029