Mitochondrial origin-binding protein UMSBP mediates DNA replication and segregation in trypanosomes

Kinetoplast DNA (kDNA) is the remarkable mitochondrial genome of trypanosomatids. Its major components are several thousands of topologically linked DNA minicircles, whose replication origins are bound by the universal minicircle sequence-binding protein (UMSBP). The cellular function of UMSBP has b...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2007-12, Vol.104 (49), p.19250-19255
Main Authors: Milman, Neta, Motyka, Shawn A, Englund, Paul T, Robinson, Derrick, Shlomai, Joseph
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biochemistry, Molecular Biology
Biological Sciences
Cell culture techniques
Cell cycle
Cell extracts
Cell growth
Cell lines
Cell nucleus
Cell Nucleus Division - genetics
Cell separation
Cellular Biology
Chromosome Segregation - genetics
Deoxyribonucleic acid
DNA
DNA Replication - genetics
DNA-Binding Proteins - antagonists & inhibitors
DNA-Binding Proteins - genetics
DNA-Binding Proteins - physiology
Fluorescence
Genome, Protozoan
Kinetoplast DNA
Life Sciences
Microbiology and Parasitology
Mitochondria - metabolism
Mitosis - genetics
Ploidies
Proteins
Protozoan Proteins - antagonists & inhibitors
Protozoan Proteins - genetics
Protozoan Proteins - physiology
Ribonucleic acid
RNA
RNA Interference
Trypanosoma brucei
Trypanosoma brucei brucei - genetics
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Summary:Kinetoplast DNA (kDNA) is the remarkable mitochondrial genome of trypanosomatids. Its major components are several thousands of topologically linked DNA minicircles, whose replication origins are bound by the universal minicircle sequence-binding protein (UMSBP). The cellular function of UMSBP has been studied in Trypanosoma brucei by using RNAi analysis. Silencing of the trypanosomal UMSBP genes resulted in remarkable effects on the trypanosome cell cycle. It significantly inhibited the initiation of minicircle replication, blocked nuclear DNA division, and impaired the segregation of the kDNA network and the flagellar basal body, resulting in growth arrest. These observations, revealing the function of UMSBP in kDNA replication initiation and segregation as well as in mitochondrial and nuclear division, imply a potential role for UMSBP in linking kDNA replication and segregation to the nuclear S-phase control during the trypanosome cell cycle.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0706858104