TAFII mutations disrupt Dorsal activation in the Drosophila embryo

In this study, we present evidence that the Dorsal activator interacts with limiting amounts of the TFIID complex in the Drosophila embryo. In vitro transcription reactions and protein binding assays implicate the TAF II 110 and TAF II 60 subunits of the TFIID complex in contributing to Dorsal-media...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1998-11, Vol.95 (23), p.13483-13488
Main Authors: Zhou, J, Zwicker, J, Szymanski, P, Levine, M, Tjian, R
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Drosophila - embryology
Drosophila - genetics
Drosophila Proteins
Embryos
Gene Expression Regulation, Developmental
Genetics
Insects
Mutation
Nuclear Proteins - genetics
Phosphoproteins - genetics
Proteins
Transcription Factor TFIID
Transcription Factors
Transcription Factors, TFII - genetics
Transcriptional Activation
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Summary:In this study, we present evidence that the Dorsal activator interacts with limiting amounts of the TFIID complex in the Drosophila embryo. In vitro transcription reactions and protein binding assays implicate the TAF II 110 and TAF II 60 subunits of the TFIID complex in contributing to Dorsal-mediated activation. Mutations in TAF II 110 and TAF II 60 result in altered patterns of snail and twist transcription in embryos derived from dl /+ females. These results suggest that TAF II s contribute to the activation of transcription in vivo and support the hypothesis that subunits of TFIID may serve as targets of enhancer binding proteins.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.95.23.13483