General requirement for RNA polymerase II holoenzymes in vivo

Yeast RNA polymerase II holoenzymes have been described that consist of RNA polymerase II, a subset of general transcription factors, and nine SRB regulatory proteins. The feature that distinguishes the RNA polymerase II holoenzymes from other forms of RNA polymerase II in the cell is their tight as...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1995-05, Vol.92 (10), p.4587-4590
Main Authors: Thompson, C.M. (Yale Medical School, New Haven, CT.), Young, R.A
Format: Article
Language:English
Subjects:
ARN MENSAJERO
ARN MESSAGER
Base Sequence
Biochemistry
Cellular biology
Enzymes
Fungal Proteins - biosynthesis
Gene Expression
General transcription factors
Genes
Genetic mutation
Half lives
Mediator Complex
Messenger RNA
Molecular Sequence Data
Mutagenesis
Oligonucleotide Probes
Oligonucleotides
Polymerase Chain Reaction
PROTEINAS
PROTEINE
RNA
RNA Polymerase II - biosynthesis
RNA Polymerase II - genetics
RNA Polymerase II - metabolism
RNA, Messenger - biosynthesis
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae Proteins
Sequence Deletion
Temperature
Transcription Factors - biosynthesis
Transfer RNA
TRANSFERASAS
TRANSFERASE
Yeast
Yeasts
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Yeast RNA polymerase II holoenzymes have been described that consist of RNA polymerase II, a subset of general transcription factors, and nine SRB regulatory proteins. The feature that distinguishes the RNA polymerase II holoenzymes from other forms of RNA polymerase II in the cell is their tight association with SRB proteins. We investigated the fraction of genes that require SRB proteins in vivo by examining the effect of temperature-sensitive mutations in SRB genes on transcription by RNA polymerase II. Upon transfer to the restrictive temperature, there is a rapid and general shutdown of mRNA synthesis in srb mutant cells. These data, combined with the observation that essentially all of the SRB protein in cells is tightly associated with RNA polymerase II molecules, argue that SRB-containing holoenzymes are the form of RNA polymerase II recruited to most promoters in the cell.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.92.10.4587