Production, Optimisation and Partial Characterisation of Enzymes from Filamentous Fungi Using Dried Forage Cactus Pear as Substrate

Purpose The forage cactus pear ( Opuntia spp. and Nopalea spp.) has been sub-utilised, mostly, for ruminant feed during long periods of drought. In this study, Nopalea cochenillifera was investigated as an alternative substrate for the production of xylanases and β-glucosidases by Aspergillus niger...

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Bibliographic Details
Published in:Waste and biomass valorization 2018-04, Vol.9 (4), p.571-579
Main Authors: dos Santos, Tamires Carvalho, dos Santos Reis, Nadabe, Silva, Tatielle Pereira, Bonomo, Renata Cristina Ferreira, Aguiar-Oliveira, Elizama, de Oliveira, Julieta Rangel, Franco, Marcelo
Format: Article
Language:English
Subjects:
Aspergillus niger
Cactaceae
Cacti
Cultivation
Drought
Engineering
Environment
Environmental Engineering/Biotechnology
Enzymatic activity
Enzymes
Experimental design
Fermentation
Fungi
Glucosidase
Industrial Pollution Prevention
Microorganisms
Nopalea
Nopalea cochenillifera
Opuntia
Original Paper
pH effects
Renewable and Green Energy
Response surface methodology
Rhizopus
Shelf life
Solid state fermentation
Stability analysis
Storage stability
Substrates
Temperature
Waste Management/Waste Technology
Water activity
Xylanase
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Summary:Purpose The forage cactus pear ( Opuntia spp. and Nopalea spp.) has been sub-utilised, mostly, for ruminant feed during long periods of drought. In this study, Nopalea cochenillifera was investigated as an alternative substrate for the production of xylanases and β-glucosidases by Aspergillus niger and Rhizopus sp. Methods The solid-state fermentation of N. cochenillifera by Aspergillus niger and Rhizopus sp. were optimised for xylanases and β-glucosidases production using a central composite face-centered (CCF) experimental design and response surface methodology (RSM), considering the variables water activity, temperature and time. The enzymes obtained were also evaluated for their pH, temperature and storage stability. Results The theoretical optimum cultivation conditions were an initial water activity of 0.865 at 30 °C for 72 h. The equivalent enzymatic activities (U g −1 ) obtained were 355.56 ( A. niger xylanase), 3456.91 ( A. niger β-glucosidase), 204.57 ( Rhizopus sp. xylanase) and 1630.07 ( Rhizopus sp. β-glucosidase). The obtained enzymes presented good stability (~80%) at
ISSN:1877-2641
1877-265X
DOI:10.1007/s12649-016-9810-z