An Investigation of Protective Effects of Litium Borate on Blood and Histopathological Parameters in Acute Cadmium-Induced Rats

This study was carried out to determine the protective effects of lithium borate (LTB) on blood parameters and histopathological findings in experimentally induced acute cadmium (Cd) toxicity in rats. Twenty-eight male Wistar albino rats were used, weighing 200–220 g, and they were randomly divided...

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Bibliographic Details
Published in:Biological trace element research 2018-04, Vol.182 (2), p.287-294
Main Authors: Yildirim, Serkan, Celikezen, Fatih Caglar, Oto, Gökhan, Sengul, Emin, Bulduk, Mehmet, Tasdemir, M, Ali Cinar, D.
Format: Article
Language:English
Subjects:
Alanine
Alanine transaminase
Albinism
Alkaline phosphatase
Animal tissues
Aspartate aminotransferase
Biochemistry
Biomedical and Life Sciences
Biotechnology
Blood
Blood cells
C-reactive protein
Cadmium
Control
Degeneration
Epithelium
Erythrocytes
Heart
Hematocrit
Hemoglobin
Hemorrhage
Hepatitis
Histopathology
Hyperemia
Infiltration
Ketamine
Kidneys
Leukocytes
Life Sciences
Lithium
Lithium borates
Liver
Lymphocytes
Monocytes
Necrosis
Nutrition
Oncology
Parameters
Phosphatase
Proteins
Rats
Renal cortex
Rodents
Statistical analysis
Steatosis
Tissue
Tissues
Toxicity
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Summary:This study was carried out to determine the protective effects of lithium borate (LTB) on blood parameters and histopathological findings in experimentally induced acute cadmium (Cd) toxicity in rats. Twenty-eight male Wistar albino rats were used, weighing 200–220 g, and they were randomly divided into four groups, including one control and the following three experimental groups: a Cd group (0.025 mmol/kg), a LTB group (15 mg/kg/day orally for 5 days), and a LTB + Cd group (15 mg/kg/day orally for 5 days and Cd 0.025 mmol/kg by intraperitoneal injection on the fifth day). All the rats in the study were anesthetized with ketamine at the end of the sixth day, blood was taken from their hearts, and then the rats were decapitated. The values in the control and LTB group were usually close to each other. White blood cell (WBC), neutrophil %, and C-reactive protein (CRP) levels increased in the Cd and LTB + Cd groups while lymphocyte and monocyte levels decreased in a statistically significant manner, in comparison to the other groups. It was determined that the levels of red blood cells (RBCs), hematocrit (Htc), and hemoglobin (Hb) did not change in the groups. The levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the Cd and LTB + Cd groups significantly increased, in comparison to the other groups, while the glucose, alkaline phosphatase (ALP), albumin (ALB), and total protein (TP) levels decreased. According to histopathological findings in the control and LTB groups, the liver and kidney tissues were found to have normal histological structures. In the Cd group, severe necrotic hemorrhagic hepatitis, mild steatosis, and mononuclear cell infiltration were detected in the liver. In the LTB + Cd group, degeneration and mild mononuclear cell infiltration were found in the liver. Regarding the kidney tissue in the Cd group, severe intertubular hyperemia in both kidney cortex and medulla, as well as degeneration and necrosis in the tubulus epithelium, was observed. In the LTB + Cd group, mild interstitial hyperemia and mononuclear cell infiltration was detected. Resultantly, it can be said that LTB at this dose has non-toxic effects and some beneficial effects for liver and kidney damage caused by acute Cd toxicity.
ISSN:0163-4984
1559-0720
DOI:10.1007/s12011-017-1089-9