Enhanced production of pharmaceutically important isoflavones from hairy root rhizoclones of Trifolium pratense L

These studies report the development of an efficient technique for large-scale cultivation of fast-growing hairy root culture systems for production of bioactive isoflavones. Trifolium pratense L. is an important source of pharmaceutically important isoflavones with immense health care applications....

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Published in:In vitro cellular & developmental biology. Plant 2018-02, Vol.54 (1), p.94-103
Main Authors: Kumar, M. Anil, Pammi, S. S. Sravanthi, Sukanya, M. S., Giri, Archana
Format: Article
Language:English
Subjects:
Agrobacterium
Agrobacterium rhizogenes
Antioxidants
Biochanin A
Biomedical and Life Sciences
Cancer
Cell Biology
Cell culture
Cultivation
Daidzein
Deoxyribonucleic acid
Developmental Biology
DNA
Genistein
Growth rate
Hairy root
Health care
Herbal medicine
Isoflavones
Kinases
Life Sciences
Metabolites
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant Sciences
PLANT TISSUE CULTURE
Pueraria
Seeds
Trifolium pratense
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Summary:These studies report the development of an efficient technique for large-scale cultivation of fast-growing hairy root culture systems for production of bioactive isoflavones. Trifolium pratense L. is an important source of pharmaceutically important isoflavones with immense health care applications. Trifolium pratense was transformed using different strains of Agrobacterium rhizogenes for hairy root induction and establishment of hairy root rhizoclones. Selected fast-growing rhizoclones of T. pratense were evaluated for their growth and isoflavone production. This study is the first report of stable production of isoflavones through successive culture passages from transformed hairy-root rhizoclones of T. pratense. One of the fast-growing hairy-root rhizoclones 2364A displayed significantly higher accumulation of all four pharmaceutically important isoflavones, 8.56 mg (gdw)−1 of daidzein, 2.45 mg (gdw)−1 of genistein, 15.23 mg (gdw)−1 of formononetin, and 1.10 mg (gdw)−1 of biochanin A, compared to other rhizoclones.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-017-9873-y