Importance of Phosphoinositide 3-Kinase {gamma} in the Host Defense against Pneumococcal Infection

The pivotal role of phosphoinositide 3-kinase gamma (PI3Kgamma) in leukocyte recruitment makes it an attractive target for immunomodulatory therapy. However, interfering with PI3Kgamma signaling might increase the risk of bacterial infections in humans. We hypothesized that deletion or pharmacologic...

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Bibliographic Details
Published in:American journal of respiratory and critical care medicine 2007-05, Vol.175 (9), p.958
Main Authors: Maus, Ulrich A, Backi, Myriam, Winter, Christine, Srivastava, Mrigank, Schwarz, Matthias K, Ruckle, Thomas, Paton, James C, Briles, David, Mack, Matthias, Welte, Tobias, Maus, Regina, Bohle, Rainer M, Seeger, Werner, Rommel, Christian, Hirsch, Emilio, Lohmeyer, Jurgen, Preissner, Klaus T
Format: Article
Language:English
Subjects:
Animals
Bacterial infections
Bone marrow
Cytotoxicity
Edema
Experiments
Kinases
Lavage
Leukocytes
Lungs
Neutrophils
Pathogens
Pneumonia
Proteins
Rheumatoid arthritis
Streptococcus infections
Virulence
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Summary:The pivotal role of phosphoinositide 3-kinase gamma (PI3Kgamma) in leukocyte recruitment makes it an attractive target for immunomodulatory therapy. However, interfering with PI3Kgamma signaling might increase the risk of bacterial infections in humans. We hypothesized that deletion or pharmacologic inhibition of PI3Kgamma would impair the lung inflammatory response to the prototypic gram-positive bacterial pathogen Streptococcus pneumoniae. PI3Kgamma knockout (KO) and wild-type mice were infected with S. pneumoniae or challenged with the pneumococcal virulence factor pneumolysin (PLY), and inflammatory leukocyte recruitment, bacterial pathogen elimination, and resolution/repair processes were determined. PI3Kgamma KO mice challenged with PLY responded with lung edema and neutrophilic alveolitis, but showed a drop in alveolar macrophages and failed to recruit exudate macrophages when compared with wild-type mice. S. pneumoniae-infected PI3Kgamma KO mice and wild-type mice pretreated with the pharmacologic inhibitor AS-605240 recruited similar numbers of neutrophils but substantially fewer exudate macrophages into their lungs than control animals. They also displayed a significantly reduced lung pneumococcal clearance and showed an impaired resolution/repair process, leading to progressive pneumococcal pneumonia. PI3Kgamma gene deletion or pharmacologic inhibition of PI3Kgamma leads to perturbations of critical innate immune responses of the lung to challenge with S. pneumoniae. These data are of clinical relevance for the treatment of chronic inflammatory diseases where pharmacologic inhibition of PI3Kgamma signaling to attenuate effector cell recruitment may have implications for innate immune surveillance of remote organ systems.
ISSN:1073-449X
1535-4970
DOI:10.1164/rccm.200610-1533OC